Protocols books specializing in measuring free radical and antioxidant biomarkers began to be published in 1998. Many of these methods are currently finding use in diagnostic medicine. Advanced Protocols for Oxidative Stress I coversthe field of oxidative stress with state of the art technology to utilize in research, contributed by an international panel of experts renowned for developing new procedures and methods. Included are sections on reactive oxygen and nitrogen species techniques, antioxidant technology and application, methods for analyzing gene expression, the exciting new area of oxidative stress and stem cell differentiation and specific biostatistical evaluation of biomarkers. This volume presents the current high-tech methodologies and provides a perspective on the diversity of applications in the ever-emerging field of free radical reactions and antioxidants. Describes novel probes for detecting ROS and RNS Provides theoretical models to predict structural features of polyphenol antioxidants Provides automated assays for ROS metabolites and antioxidant capacity Use of HPLC-nanoHPLC-ESR-MS-ESI-ECD and high-voltage capillary electrophoresis Shows how correlative transmission microscopy allows for in-situ visualization of localized sites of oxidative stress INDICE: From the contents Novel Designed Probes for the Characterization of Oxidative Stress in Biological Fluids, Cell and Tissues.- A Rapid and Selective Mass Spectroscopic Method for the Identification of Nitrated Proteins.- AnEasy and Reliable Automated Method to Estimate Oxidative Stress in the Clinical Setting.- Correlative Transmission Microscopy: Cytochemical Localization and Immunocytochemical Localization in Studies of Oxidative and Nitrosative Stress.- Detection of Specifically Oxidized Apolipoproteins in Oxidized HDL.- HighPerformance Liquid Chromatography / Electron Spin Resonance / Mass Spectroscopy Analysis of Lipid-Derived Radicals.- EPR Spin-Trapping and Nano LC MS/MS Techniques for DEPMPO/ OOH and Immunospin-trapping with Anti-DMPO Antibody in Mitochondrial Electron Transfer System.- Determination of High Mitochondrial Membrane Potential in Spermatazoa loaded with the Mitochondrial Probe 5, 5’, 6, 6’- tetrachloro-1, 1’, 3, 3’-tetraethylbenzimazoyolyl-carbocyanine iodide ( JC-! ).
- ISBN: 978-1-60327-218-6
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 380
- Fecha Publicación: 01/10/2008
- Nº Volúmenes: 1
- Idioma: Inglés