This new volume of Methods in Cell Biology looks at methods for analyzing centrosomes and centrioles. Chapters cover such topics as methods to analyze centrosomes, centriole biogenesis and function in multi-ciliated cells, laser manipulation of centrosomes or CLEM, analysis of centrosomes in human cancers and tissues, proximity interaction techniques to study centrosomes, and genome engineering for creating conditional alleles in human cells. Covers sections on model systems and functional studies, imaging-based approaches and emerging studiesChapters are written by experts in the fieldCutting-edge material INDICE: 1. Chemical inhibition of centriole assembly Karen Oegema2. Methods to analyse centrosomes in fly and mouse brains Renata Basto3. Methods to analyse centrosomes in Drosophila embryos-antibody injections James Wakefield4. Using chicken DT40 cells to functionally analyse centrosomes Fanni Gergely5. High resolution microscopy- SIM to characterise PCM organisation Vito Mennella6. Centriole biogenesis and function in multi-ciliated cells Brian Mitchell7. Tomography analysis of basal bodies from mouse trachea Delphine Delacour8. Centrosome purification from adherent and non-adherent cell lines Anne-Marie Tassin and Delphine Gogendeau 9. Purification and structural characterization of g-tubulin containing complexes Justin M. Kollman10. Laser manipulation of centrosomes or CLEM Jadranka Loncarek11. In vitro analysis of PCM assembly Jeffrey B. Woodruff12. Analysis of centrosomes in human cancers-in humna tissues Stefan Duensing13. Spindle pole body in F. yeast Anne Paoletti14. Analysis of centrosomes in organotypic cultures Susana A. Godinho15. Y2H screens of Drosophila centrosome genes Naser M. Rusan and Brian Galletta 16. Centrosomes in ascidian embryos Alex McDougall17. Proximity interaction techniques to study centrosomes Tim Stearns and Elif Nur Firat-Karalar 18. EM of basal bodies and transition zone in sensory cilia Alexander Dammerman19. Genome engineering for creating conditional alleles in human cells Andrew Jon Holland20. Using S2 cells for screening genes involved in centrosome biogenesis Jeroen Daubelaere21. Centrosomes in neuroblasts Cayetano Gonzalez
- ISBN: 978-0-12-802449-2
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 362
- Fecha Publicación: 01/08/2015
- Nº Volúmenes: 1
- Idioma: Inglés