Macromolecular drugs hold the promise of becoming new therapeutics for several major disorders, including cancer and cardiovascular disease. This incredible potential is explored in Macromolecular Drug Delivery, a volume which gives a wide-ranging overview of contemporary methods used in the field, and which addresses the limitations presented by a lack of safe and efficient drug delivery strategies. Chapters offer information on both in vitro and in vivo methodsof macromolecular delivery, thus appealing to a broad scientific audience. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting andavoiding known pitfalls. Comprehensive and cutting-edge, Macromolecular Drug Delivery offers a platform for interdisciplinary collaboration, which should provide opportunities for new discoveries at the interface between disciplines.Provides cutting edge methods for in vitro and in vivo studies of nucleic acid based drugs Presents detailed protocols for preparation of non-viral drug delivery vehicles and artificial membrane models Includes a comprehensive description of retrieval of internalizing antibodies from phage display libraries INDICE: From the contents Developments in Macromolecular Drug Delivery; Review Chapter.-Synthetic vs. Natural/Biodegradable Polymers for Delivery of shRNA Based Cancer Therapies; Review chapter.- siRNA and DNA Transfer to CulturedCells.- Poly(ß-amino esters): Procedures for Synthesis and Gene Delivery.- Systemic Delivery and Preclinical Evaluation of Nanoparticles Containing Antisense Oligonucleotides and siRNAs.- Peptide-based Delivery of Steric-block PNA Oligonucleotides.- Characterizing Peptide Mediated DNA Internalization in Human Cancer Cells.- Selection and Characterization of Antibodies from Phage DisplayLibraries against Internalizing Membrane Antigens.- Artificial Membrane Models for the Study of Macromolecular Delivery.- Enhanced Delivery of Macromolecules into Cells by Electroendocytosis.- In Vitro Systems for Studying EpithelialTransport of Macromolecules.
- ISBN: 978-1-58829-999-4
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 240
- Fecha Publicación: 01/10/2008
- Nº Volúmenes: 1
- Idioma: Inglés