The development of molecular genotyping methods has revolutionized the possibility for classification of microorganisms at the sub-species level. This investigation of species diversity is crucial for deciding the molecular relatedness of isolates for epidemiological studies. In Molecular Epidemiology of Microorganisms: Methods and Protocols, readers will find readily reproducible methods for determining the molecular epidemiology of microorganisms, all of which provide effective discrimination of unrelated strains. This volume covers a wide range of techniques which can be easily applied to the investigation of transmissible diseases, directing researchers towards the most effective methods based on the particular information to be obtained. Describing both traditional and novel techniques, expert researchers present a series of methods-based chapters with applications to some of the most important microbes. Includes cutting-edge methods for the epidemiology of microorganisms Provides a comparative overview of methods best adapted to species diversity Includes tools for advanced data analyses and interpretation INDICE: From the contents Microbial Molecular Epidemiology: an Overview.- Multilocus Enzyme Electrophoresis for Parasites and other Pathogens.- Plasmid Replicon Typing.- The Application of Randomly Amplified DNA Analysis in the Molecular Epidemiology of Microorganisms.- Use of Repetitive Element Palindromic-PCR (rep-PCR) for the Epidemiological Discrimination of Foodborne Pathogens.-Pulsed-Field Gel Electrophoresis for Molecular Epidemiology of Food Pathogens.- Molecular Genotyping of Microbes by Multilocus PCR and Mass Spectrophotometry: a New Tool for Hospital Infection Control and Public Health Surveillance.-Amplified Fragment Length Polymorphism Analysis.- Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) for the Genotyping of Bacterial Pathogens.- Spoligotyping for Molecular Epidemiology of the Mycobacterium tuberculosis Complex.- Multilocus Sequence Typing.
- ISBN: 978-1-60327-998-7
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 290
- Fecha Publicación: 01/05/2009
- Nº Volúmenes: 1
- Idioma: Inglés