Cell-based assays for high-throughput screening: methods and protocols

As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes evermore pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology™ series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls. Provides an easily accessible reference volume for cell-based phenotypic screening written by leading researchers in the field Details the process of turning a biological observation into a high-throughput screen Covers a wide range of cell-based assay formats and systems INDICE: Introduction: Cell-based Assays for High-throughput Screening.- High-throughput Screening of Model Bacteria.- Cell-based Assays to Probe the ERKMAP Kinase Pathway in Endothelial Cells.- Large-scale Small-molecule Screen Using Zebrafish Embryos.- Whole-animal High-throughput Screens: The C. elegans Model.- Whole-organism Screening: Plants.- Fluorescence-based Assays.- Reporter-gene Assays.- Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-restoration Assay.- Assay for Isolation of Inhibitors of Her2-kinase Expression.- ImageJ: A Primer.- High-content Screening: Flow Cytometry Analysis.- High-throughput Real-time PCR for Detection of Gene-expression Levels.- Interpretation of Uniform-well Readouts.- Extracting Rich Information from Images.

• ISBN: 978-1-60327-544-6
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 250
• Fecha Publicación: 01/11/2008
• Nº Volúmenes: 1
• Idioma: Inglés