Bacteriophages: methods and protocols v. 2 Molecular and applied aspects

Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design volume 2: Molecular and Applied Aspects examines a multitude of topics,including the bacteriophage genomics, metagenomics, transcriptomics, and proteomics, along with applied bacteriophage biology. Written in the highly successful Methods in Molecular Biology™ series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tipson troubleshooting and avoiding known pitfalls. Examines tried-and-true methods along with the most cutting-edge protocols Ideal for both professionals andnewcomers due to convenient, easy-to-use style Designed for modular use, providing a potential wide array of techniques based on the combination of the methods described INDICE: From the contents Preparation of Bacteriophage Lysates and Pure DNA.- Approaches to the Compositional Analysis of DNA.- Determination of Bacteriophage Genome Size by Pulsed-Field Gel Electrophoresis.- Preparation of a Phage DNA Fragment Library for Whole Genome Shotgun Sequencing.- PCR and Partial Sequencing of Bacteriophage Genomes.- In Silico Identification of Genes in Bacteriophage DNA.- Determining DNA Packaging Strategy by Analysis of the Termini of the Chromosomes in Tailed-Bacteriophage Virions.- In silico Characterization of DNA Motifs with Particular Reference to Promoters and Terminators.- Molecular Phylogenetics: Testing Evolutionary Hypotheses.- Preparation of RNA from Bacteria Infected with Bacteriophages: A Case Study from the Marine Unicellular Synechococcus sp. WH7803 Infected by Phage S-PM2.- Quantification of Host and Phage mRNA Expression during Infection Using Real-Time PCR.

• ISBN: 978-1-60327-564-4
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 320
• Fecha Publicación: 01/11/2008
• Nº Volúmenes: 1
• Idioma: Inglés