Methods in Enzymology, Volume 650 continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Biophysical methods to study lanthanide-protein interactions, Genetically encoded sensors to study lanthanide biology, Spectrophotometric methods to determine the stability constants of lanthanide-macromolecule complexes, Lanthanide-based probes for amino acid modifications, In vitro selection and application of lanthanide-dependent DNAzymes, LRET biosensors for imaging protein interactions in living cells, Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor, EPR spectroscopy of lanthanides, Macromolecular crystallography for f-element complex characterization, and much more. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology series INDICE: 1. Biophysical methods to study lanthanide-protein interactions 2. Genetically encoded sensors to study lanthanide biology 3. Spectrophotometric methods to determine the stability constants of lanthanide-macromolecule complexes 4. Lanthanide-based probes for amino acid modifications 5. In vitro selection and application of lanthanide-dependent DNAzymes 6. LRET biosensors for imaging protein interactions in living cells 7. Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor 8. EPR spectroscopy of lanthanides 9. Macromolecular crystallography for f-element complex characterization 10. Characterization of lanthanoid binding proteins using NMR spectroscopy 11. Infrared spectroscopy probes ion binding geometries 12. Isolation and characterization of new lanthanide-dependent methylotrophs 13. Mass spectrometry-based approaches to study lanthanides in the phyllosphere 14. Transposon mutagenesis for methylotrophic bacteria 15. Yttrium-86 PET imaging 16. Gadolinium spin labels 17. Applications of scandium complexes 18. Activity assays of methanol dehydrogenases 19. Protein expression in M. extorquens 20. Expression, purification and properties of enzymes involved in lanthanide-dependent alcohol oxidation: XoxF4, XoxF5, ExaF/PedH, XoxG4
- ISBN: 978-0-12-823856-1
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 01/04/2021
- Nº Volúmenes: 1
- Idioma: Inglés