Since the discovery of microRNAs, developmental biologists have striven to understand the role of miRNAs in development and disease. MicroRNAs in Development: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activityof miRNAs in various organisms, and it concludes with a section that outlinesdifferent methods to identify and validate miRNA targets in animals and plants. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, MicroRNAs in Development: Methods and Protocols serves as a practical guide for scientists of all backgrounds and conveys the appropriate sense of fascination associated with this vital field of research. Includes cutting-edge methods and protocols. Provides step-by-step detail essential for reproducible results. Contains key notes and implementation advice from the experts. INDICE: Mimicry Technology: Suppressing Small RNA Activity in Plants. Experimental Validation of MicroRNA Targets using a Luciferase Reporter System. Experimental Identification of microRNA Targets by Immunoprecipitation of Argonaute Protein Complexes. Comprehensive Identification of miRNA Target Sites in Live Animals. Target Validation of Plant microRNAs.- A High-throughput Sequencing-based Methodology to Identify All Uncapped and Cleaved RNA Molecules in Eukaryotic Genomes.
- ISBN: 978-1-61779-082-9
- Editorial: Humana Press
- Encuadernacion: Cartoné
- Páginas: 200
- Fecha Publicación: 29/04/2011
- Nº Volúmenes: 1
- Idioma: Inglés