Replacing animal models: a practical guide to creating and using culture-based biomimetic alternatives

Over the last decade, in vitro models have become more sophisticated and are at a stage where they can provide an effective alternative to in vivo experiments. Replacing Animal Models provides scientists and technicians with a practical, integrated guide to developing culture-based alternatives to in vivo experiments.The book is neither political nor polemical: it is technical, illustrating by example how alternatives can be developed and used and providing useful advice on developing others. After looking at the reasons for and potential benefits of alternatives to animal experiments, the book covers a range of methods and examples emphasising the design considerations that went into each system. The chapters also include 'case studies' that illustrate the ways in which culture models can be used to answer a range of important biological questions of direct relevance to human development, physiology, disease and healing.The thesis of this book is not that all animal experimentation can be replaced, now or in the near future, by equally effective or superior alternatives. Rather, the premise is that there is substantial opportunity, here and now, to do some common types of experiment better in vitro than in vivo, and that doingso will result in both scientific and ethical gains. INDICE: Contributors, viiPreface, xiSection 1 Introductory Material1 Potential Advantages of Using Biomimetic Alternatives, 3Jamie Davies2 Overview of Biomimetic Alternatives, 13Jamie DaviesSection 2 Culture Methods3 Pancreatic Islets, 23Eli C Lewis4 Endometrial Organoid Culture, 35Merja Blauer5 Modelling Lymphatic and Blood Capillary Patterning, 45Francoise Bruyere, Catherine Maillard, Charlotte Erpicum and Agnes Noel6 Precision-cut Lung Slices (PCLS), 57Christian Martin and Stefan Uhlig7 Human Colon Tissue in Organ Culture, 69Michael K Dame and James Varani8 Fetal Organ Culture, 81Jamie Davies9 Design of a Mechanical Loading Device to Culture Intact Bovine Spinal Motion Segments under Multiaxial Motion, 89Jochen Walser, Stephen John Ferguson and Benjamin Gantenbein-Ritter10 Magnetic Assembly of Tissue Surrogates, 107Chien-Yu Fu and Hwan-YouChang11 Assembly of Renal Tissues by Cellular Self-organization, 115Mathieu UnbekandtSection 3 Case Studies of Use12 Hierarchical Screening of Pathways: Using Cell and Organ Cultures to Reduce use of Transgenic Mice, 125Guangping Taiand Jamie Davies13 Lung Organoid Culture to Study Responses to Viruses, 137Wenxin Wu, J Leland Booth and Jordan P Metcalf14 Organ-cultured Human Skin for the Study of Epithelial Cell Invasion of Stroma, 151James Varani15 Organotypic Mandibular Cultures for the Study of Inflammatory Bone Pathology, 159Alastair J Sloan, Sarah Y Taylor and Emma L Smith16 Three-dimensional, High-density andTissue Engineered Culture Models of Articular Cartilage, 167Ali Mobasheri, Sara Kelly, Abigail L. Clutterbuck, Constanze Buhrmann and Mehdi Shakibaei17 Concluding Remarks, 193Jamie DaviesAppendix 1 Sources of funding for development of culture-based alternatives, 195Appendix 2 Databases and web-based discussions relevant to development of alternatives, 197Index, 199

• ISBN: 978-1-119-94068-5
• Editorial: John Wiley & Sons
• Encuadernacion: Rústica
• Páginas: 216
• Fecha Publicación: 27/03/2012
• Nº Volúmenes: 1
• Idioma: Inglés