The Zebrafish: Genetics, Genomics, and Transcriptomics

The Zebrafish: Genetics, Genomics, and Transcriptomics, Fourth Edition, is the latest volume in the Methods in Cell Biology series that looks at methods for the analysis of genetics, genomics, and transcriptomics of Zebrafish. Chapters cover such topics as gene-trap mutagenesis, genetic Screens for mutations, gene editing in zebrafish, homologous gene targeting, genome-wide RNA tomography, and developmental epigenetics and the zebrafish interactome. Covers sections on model systems and functional studies, imaging-based approaches, and emerging studiesPresents chapters written by experts in the fieldContains cutting-edge material on the topic INDICE: 1. Generating Conditional Mutations in Zebrafish Using Gene-trap Mutagenesis   Wenbiao Chen2. Tol2-mediated Transgenesis, Gene Trapping, Enhancer Trapping and the Gal4-UAS System  Koichi Kawakami3. Genetic Screens for Mutations Affecting Adult Traits and Parental-effect Genes   Mary Mullins4. Targeted Candidate Gene Screens Using CRISPR/Cas9 Technology   Adam C. Miller and Cecilia Moens 5. TALEN and CRISPR Enhanced DNA Homologous Recombination for Gene Editing in Zebrafish   Shuo Lin6. Advances in Homologous Gene Targeting in the Zebrafish   David Grunwald7. The Zebrafish Genome Editing Toolkit   Stephen Ekker8. Clonal Analysis of Gene Loss-of-function and Tissue-specific Gene Deletion in Zebrafish via CRISPR/Cas9 Technology   Filippo Del Bene9. Tissue-specific Gene Targeting using CRISPR/Cas9  Julien Ablain10. The Q Transcriptional Regulatory System for Zebrafish Transgenesis   Marnie Halpern11. Advanced Zebrafish Transgenesis with Tol2 and Application for Cre/lox Recombination Experiments  Christian Mosimann12. Transgenic Zebrafish Using Transposable Elements   Stephen Ekker13. Method for Somatic Cell Nuclear Transfer in Zebrafish   Jose Cibelli14. Genome-wide RNA Tomography   Jeroen Bakkers15. Analyzing the Transcriptome of Zebrafish Hair Cells by TU-tagging   Teresa Nicolson16. Expressed Repetitive Elements for RT-qPCR Normalization   H. William Detrich III and Andy Willaert 17. Experimental Approaches for Studying Splicing Variation in Zebrafish   Graham J. Lieschke, Joan Heath and Cristina Keightley 18. PICCORO? - A Novel Technique for Manipulating the Activity of Transcription Factors with Blue Light   Mikiko Tanaka and Shinji Masuda 19. DNA Methylation Profiling in Zebrafish   Bradley R. Cairns20. Chromatin Immunoprecipitation and Assays for Chromatin State in Zebrafish Cells   Yi Zhou and Leonard I. Zon 21. Developmental Epigenetics and the Zebrafish Interactome  José Luis Gómez-Skarmeta22. Epigenetic Regulation of Hematopoietic Stem Cell Development   Todd Evans and Mary Goll 23. A Scientist's Guide for Submitting Data to ZFIN  Monte Westerfield and Doug Howe 24. Advances in Zebrafish Husbandry and Management   Christian Lawrence25. Aquaculture and Husbandry at the Zebrafish International Resource Center   Zoltan Marton Varga26. Health Monitoring and Disease Prevention at the Zebrafish International Resource Center   Zoltan Marton Varga and Katy Murray

• ISBN: 978-0-12-803474-3
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 362
• Fecha Publicación: 01/07/2016
• Nº Volúmenes: 1
• Idioma: Inglés