The multi-authored book contains modern information on all major aspects related to the formation, stability and biological consequences of the asymmetrical organization of lipids in cell membranes. It introduces the phenomenon of membrane asymmetry, describes transmembrane distribution of lipids in biologicalmembranes, represents methods for the measurement of the transmembrane lipid motion, emphasizes the role of flippases, discusses biological functions associated with lipid asymmetry and outlines correlations between lipid asymmetry and human diseases such as thrombosis, cancer, and apoptosis. Many different techniques that are used to measure the rate of transmembrane movement of lipidsin biological systems and in model systems will be described in the book (seeTable of contents, Part I and II: Use of Electron Spin Resonance with spin-labeled lipids or fluorescent lipids; analysis of vesicle or cell shape changes to determine lipid flip-flop rates). Relevance and use of probed lipids will be discussed extensively with many examples. Examples of flippase activity willbe described in whole cells and also in reconstituted systems wherever possible. The molecular biology implied in the expression of the candidate flippasesand the purification steps will be included although they are generally classical techniques.Philippe F. Devaux received his Ph.D. in physics at the University of Paris in 1969. He then served as a postdoctoral fellow at Stanford University's chemistry department before returning to the University of Paris in 1972 to fund a biophysics group within the physics department for the investigation of lipid mobility and lipid-protein interactions. In 1981 he became Professor of Physics at the Paris 7 University, where he currently teaches courses on molecular and cellular biophysics and physics applied to biology and medicine. He is the author of over 184 scientific papers, three books, and is an editor of the European Biophysics Journal.
- ISBN: 978-0-470-38845-7
- Editorial: John Wiley & Sons
- Encuadernacion: Cartoné
- Páginas: 400
- Fecha Publicación: 05/05/2010
- Nº Volúmenes: 1
- Idioma: Inglés