Drosophila Cell Culture, Second Edition includes comprehensive coverage of cell lines, methods for creating cell lines, methods for genome engineering, and the use of cell lines for genome wide rNAi screens. This publication summarizes over thirty years of experience in the handling of in vitro cultured Drosophila cells alongside recent methods and functional screens. Early and experienced researchers studying drosophila in developmental biology, genetics, neuroscience, and across the biological and biomedical sciences will benefit from this expert knowledge. Offers full coverage of cell lines and primary culturesProvides a go-to resource for methods and studies completed with drosophila cells in culturePresents a wide spectrum of experimental techniques INDICE: 1. Composition of the Body Fluid of Drosophila and the Design of Culture Media for Drosophila Cells 2. Primary Cell Cultures of Drosophila Embryonic Cells 3. Continuous Drosophila Cell Lines Established In Vitro 4. Cells or Tissues In Course of Differentiation 5. Hormonal Control of The Development of Insects: Implications In Organ or Cell Cultures of Drosophila 6. Gene Transfer into Cultured and Applications 7. Rnai and Crispr (Cas9, Cas9-Act, Cas9-Repressor) Methods to Perturb Gene Function 8. Functional Genomics Screens Using Rnai, Crispr, and Orf Overexpression
- ISBN: 978-0-12-809473-0
- Editorial: Academic Press
- Encuadernacion: Rústica
- Páginas: 768
- Fecha Publicación: 01/11/2017
- Nº Volúmenes: 1
- Idioma: Inglés