The ability to detect and quantify apoptosis, to understand its biochemistry,and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Provides an easily accessible reference volume for methods to investigate the critical steps in apoptotic cell death written by leading researchers in the field Details the process of how to induce, measure and differentiate apoptosis in various models Covers a wide range of model systems for apoptosis detection from cell-based assays to measurements at the level of organ or organism INDICE: From the contents Analyzing Caspase Activation and Caspase Activity in Apoptotic Cells.- Flow Cytometry-Based Apoptosis Detection.- Live to DeadCell Imaging.- Detection of Apoptosis in Tissue Sections.- Detection of Apoptosis in Cell-Free Systems.- Methods to Analyze Cellular Necroptosis.- Detection of Cell Death by Autophagy.- Methods to Analyze Transglutamination of Proteins Involved in Apoptosis.- Methods to Analyze S-nitrosylation of Proteins Involved in Apoptosis.- Application of in vivo EPR for Tissue pO2 and Redox Measurements.- Assays to Measure p53-dependent and Independent Apoptosis.- Measurement of Changes in Cdk2 and Cyclin O-Associated Kinase Activity in Apoptosis.- Fluorimetric Methods for Detection of Mitochondrial Membrane Permeabilization in Apoptosis.- Regulation of Apoptosis by the Unfolded Protein Response.- Detection of Uncoupling Protein-2 (UCP-2) as a Mitochondrial Modulator of Apoptosis.- A Multiple Approach to Analyzing the Role of MicroRNAs in Apoptosis.
- ISBN: 978-1-60327-016-8
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 360
- Fecha Publicación: 01/05/2009
- Nº Volúmenes: 1
- Idioma: Inglés