Plant virology protocols: from viral sequence to protein function

Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section ofPlant Virology Protocols, Second Edition presents the most novel methods and protocols to allow researchers more effective approaches to study plant viruses. The many subjects covered in this important new edition range from techniques for investigating viral nucleic acid sequence, protein analysis and investigation of protein function, techniques for microscopy/GFP visualization and analysis/protein tagging/generation of infectious clones and other such tools, as well as areas of genomics and interactions with host factors and plant-basedstudies. Plant Virology Protocols, Second Edition: From viral sequence to protein function is indispensable to laboratory investigators studying plant viruses. Offers step by step reproducible laboratory protocols written by experts in the field of plant sciences, virology, and genomics. Focus on the novel andemerging techniques used to analyze, understand, and treat plant viruses. Covers the spectrum of plant virology protocols, from general introductions to typical plan viral proteins, techniques for investigating viral acid sequences INDICE: From the contents Plant virus interactions. Role of capsid proteins. Role of plant virus movement proteins. Multiple roles of viral replication proteins in plant RNA virus replication. Role of silencing suppressor proteins. Role of vector transmission proteins. In vivo analysis of Viral RNA translation. In vitro analysis of translational enhancer. Identification of plant virus IRES. Analysis of geminivirus DNA replication by 2-D gel. Begomoviruses: Molecular cloning and identification of replication origin. Analysis of viroid replication. Biochemical Analyses of the Interactions Between Viral Polymerases and RNAs. In situ detection of plant viruses and virus-specific products. Detection of siRNAs and miRNAs. Cloning of short interfering RNAs from virus infected plants. Solution structure probing of RNA structures. RNA encapsidation assay.

• ISBN: 978-1-58829-827-0
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 816
• Fecha Publicación: 01/05/2008
• Nº Volúmenes: 1
• Idioma: Inglés