Tumor Immunology and Immunotherapy - Molecular Methods
Galluzzi, Lorenzo
Rudqvist, Nils-Petter
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Authority of the authorsComprehensiveness of the bookLevel of details INDICE: 1. Droplet digital PCR for measuring circulting tumor-derived DNA Sara Valpione 2. Detection and quantification of cytosolic DNA Claire I. Vanpouille-Box 3. Methods to detect endogenous dsRNA induction and recognition Daniel De Carvalho 4. Quantification of eIF2alpha phosphorylation during immunogenic cell death Lucillia Bezu 5. Assessment of annexin A1 release during immunogenic cell death Elisa Elena Baracco 6. Luciferase-assisted detection of extracellular ATP in the course of ICD George Dubyak 7. The P2X7 receptor: structure and function Francesco Di Virgilio 8. Bead-assisted multiplex cytokine profiling by flow cytometry Jason S. Lehmann 9. Methods for testing HMGB1 release during immunogenic cell death Liwei Zhao 10. Side-by-side comparison of methods to assess HGMB1 release Michael Thomas Lotze 11. IDO1 protein detection in cells and tissues George Prendergast 12. Quantification of IDO1 enzymatic activity in normal and malignant tissues Derek A. Wainwright 13. Assessment of CD73 enzymatic activity in serum Dr Paolo Antonio Ascierto 14. Biochemical evaluation of CD73-dependent ADORA2A signaling John Stagg 15. SPR to study perforin-membrane interactions Gregor Anderluh 16. Methods to study perforin-driven membrane permeabilization Ilia Voskobolinik 17. Design of CD1d-scFV antitumor fusion proteins for redirecting the innate and adaptive immune responses to tumors Pedro Romero 18. Employing small molecules as a tool to study functionality of immunomodulatory proteins Georgius Pantouris 19. In vitro assay for the development of small molecule inhibitors of PD-1/PD-L1 Yanfeng Gao 20. Crystallographic approaches to study the interaction modes of PD-1- and CTLA4-blocking antibodies Norimichi Nomura 21. Cristallographic methods to study the TCR-antigen-MHC interaction Ashley Buckle 22. High-troughput discovery of cancer-targeting TCRs Michael Platten 23. TCR validation towards gene therapy for cancer Michael Platten 24. Assessing TCR repertoires using high-throughput TCR alpha and beta CDR3 region sequencing Jennifer Sims 25. Using the T cell receptor as a molecular barcode for tracking anti-tumor immunity and treatment response Kellie N. Smith 26. Methods to investigate the biological activity of antitumor antibodies Olivera J. Finn 27. Biomaterials for immunotherapy Anne Monette 28. Quantitative analysis of the T cell receptor repertoire Benny Chain 29. CRATES: A one-step assembly method for Class 2 CRISPR arrays Chase Beisel
- ISBN: 978-0-12-818671-8
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 01/12/2019
- Nº Volúmenes: 1
- Idioma: Inglés