An Introduction to Biotechnology: The Science, Technology and Medical Applications

An Introduction to Biotechnology is a biotechnology textbook aimed at undergraduates. It covers the basics of cell biology, biochemistry and molecular biology, and introduces laboratory techniques specific to the technologies addressed in the book; it addresses specific biotechnologies at both the theoretical and application levels.Biotechnology is a field that encompasses both basic science and engineering. There are currently few, if any, biotechnology textbooks that adequately address both areas. Engineering books are equation-heavy and are written in a manner that is very difficult for the non-engineer to understand. Numerous other attempts to present biotechnology are written in a flowery manner with little substance. The author holds one of the first PhDs granted in both biosciences and bioengineering. He is more than an author enamoured with the wow-factor associated with biotechnology; he is a practicing researcher in gene therapy, cell/tissue engineering, and other areas and has been involved with emerging technologies for over a decade. Having made the assertion that there is no acceptable text for teaching a course to introduce biotechnology to both scientists and engineers, the author committed himself to resolving the issue by writing his own. The book is of interest to a wide audience because it includes the necessary background for understanding how a technology works.Engineering principles are addressed, but in such a way that an instructor can skip the sections without hurting course contentThe author has been involved with many biotechnologies through his own direct research experiences. The text is more than a compendium of information - it is an integrated work written by an author who has experienced first-hand the nuances associated with many of the major biotechnologies of general interest today. INDICE: List of FiguresList of TablesPrefaceAbout the AuthorChapter 1: MembranesAbstract1.1 Membrane Lipids1.2 Cholesterol1.3 Membrane ProteinsQuestionsChapter 2: ProteinsAbstract2.1 Amino Acids2.2 Protein Structure2.3 The Hydrophobic Effect2.4 A Return to MembranesQuestionsChapter 3: Cellular TransportAbstract3.1 Membrane Transporters3.2 Vesicular Transporters: Endocytosis3.3 Receptor Fates3.4 Lysosomes Are for Degradation, But Are They Safe?QuestionsChapter 4: Genes: The Blueprints for ProteinsAbstract4.1 Nucleotides and Nucleic Acids4.2 From Genes to ProteinsQuestionsChapter 5: Cell GrowthAbstract5.1 The Eukaryotic Cell Cycle5.2 Growth Curves and Their Phases5.3 Mathematics of the Growth Curve5.4 Counting Cell Numbers5.5 Counting Cell Mass5.6 Scale-UpQuestionsChapter 6: Microbial KillingAbstract6.1 The Gram Stain6.2 Microbial Resistance to Killing6.3 Sterilization, Disinfection, and Sanitization6.4 Microbial Cell DeathQuestionsChapter 7: Cell Culture and the Eukaryotic Cells Used in BiotechnologyAbstract7.1 Adherent Cells Versus Nonadherent Cells7.2 Primary Cells, Cancer Cells, and Cell LinesQuestionsChapter 8: FluorescenceAbstract8.1 Stokes' Experiments8.2 Fluorophore Properties8.3 Fluorescence Detection8.4 FRETQuestionsChapter 9: Locating Transcriptional Control Regions: Deletion AnalysisAbstract9.1 An Example of Deletion AnalysisQuestionsChapter 10: Agarose GelsAbstract10.1 Application of Agarose Gels: Gel Shift10.2 Application of Agarose Gels: DNA Footprinting10.3 Application of Agarose Gels: Restriction AnalysisQuestionsChapter 11: The Polymerase Chain ReactionAbstract11.1 Melt11.2 Anneal11.3 Extend11.4 PCR Loops11.5 An Application of Traditional PCR11.6 Traditional Versus Real-Time PCR11.7 Real-Time PCRQuestionsChapter 12: Genetic EngineeringAbstract12.1 Plasmid Architecture12.2 Molecular Cloning12.3 A Single Plasmid Is Not Enough12.4 Spectrophotometry12.5 What We Have Learned so FarQuestionsChapter 13: Gene DeliveryAbstract13.1 Gene Delivery Vehicles: An Overview13.2 Gene Methods in Greater Detail13.3 Preparation of Nonviral Gene Delivery ComplexesQuestionsChapter 14: RNAiAbstract14.1 Cosuppression14.2 RNA Interference14.3 miRNAQuestionsChapter 15: DNA FingerprintingAbstract15.1 Older DNA Fingerprinting Uses RFLPs15.2 Newer DNA Fingerprinting Uses STRsQuestionsChapter 16: Fermentation, Beer, and BiofuelsAbstract16.1 Glycolysis16.2 Fermentation16.3 The Production of Beer16.4 Fermentation to Produce BiofuelsQuestionsChapter 17: Stem Cells and Tissue EngineeringAbstract17.1 Potential17.2 An Alternate View of Stem Cells17.3 Using Stem Cells17.4 Tissue Engineering and Regenerative Medicine17.5 Bioreactors17.6 Polymeric Scaffolds17.7 Bringing it all Together: A Tissue Engineering ApplicationQuestionsChapter 18: TransgenicsAbstract18.1 Ice-Minus Bacteria18.2 Bt Plants18.3 Herbicide Resistance18.4 Tomatoes18.5 Rice18.6 Terminators and TraitorsQuestionsChapter 19: Patents and LicensesAbstract19.1 Types of Patents19.2 Licenses19.3 After a License Is GrantedQuestionsIndex

• ISBN: 978-0-08-101506-3
• Editorial: Woodhead Publishing
• Encuadernacion: Rústica
• Páginas: 500
• Fecha Publicación: 30/06/2016
• Nº Volúmenes: 1
• Idioma: Inglés