Endosomes not only serve as a receiving compartment for endocytosed cargos, but also function as a donor compartment and sorting station for cargos designated to lysosomes, Golgi, or plasma membrane. In recent years, the importance of endosomes as a sorting and recycling compartment has become increasingly appreciated. As such, scientists from various fields of cell biology, membrane traffic and beyond, see the needs to communicate and learn about the methods used to investigate the dynamics and functions of endosomes. The aim of this volume is to bring together specialists from the field to contribute their expertise to a broad range of biomedical researchers interested in endosomal sorting and recycling. This volume covers the approaches to study the key components mediating the generation and transport of membrane-bounded carriers from the endosomes, and how membrane trafficking machinery is coordinated with cytoskeletons during these processes. In addition to studies carried out in mammalian cells, other model systems such as worm and yeast are also included. Covers an increasingly appreciated field in cell biologyIncludes both established and new technologies Contributed by experts in the field INDICE: 1. Imaging recycling endosome Rabs in innate immune cells Jennifer L. Stow2. Arf6-Rab5 Julie Donaldson3. Worm genetics Anbing Shi and Barth D. Grant 4. EHDs Steve Caplan5. Rab11 Rytis Prekeris6. Rabin8 BRET Wei Guo7. Rab22 recycling Guangpu Li8. Exocyst in Golgi and RE, and maybe Ral? Xiao-Wei Chen9. Rab and microtubules Bruno Goud10. Golgi lipids William J. Brown11. yeast Arf and Arf GEF Chris Fromme12. WASH, retromer Daniel Denis Billadeau13. WASH and endosome microdomains Alexis Gautreau14. ACAP1 Victor Hsu15. Arf GAP Paul Randazzo16. epithelia endosomes; transcytosis Gerard Apodaca17. AP-1 basolateral sorting Enrique Rodriguez-Boulan18. GPCR Sorting at the Multivesicular Endosomes JoAnn Trejo19. u1B Heike Fölsch20. Rab11 and mitotic spindle organization Stephen Doxsey21. GPCR signaling Manoj Puthenveedu22. EGFR degradation and recycling Alexander Sorkin
- ISBN: 978-0-12-802829-2
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 362
- Fecha Publicación: 01/10/2015
- Nº Volúmenes: 1
- Idioma: Inglés