Chemokines, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in chemokines, and includes sections on such topics as chemokine detection using receptors, tracking cellular responses to chemokines, recognition of GAG-bound chemokines, and the production of chemokine receptor complexes for structural and biophysical studies. Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers research methods in chemokinesContains sections on such topics as chemokine detection using receptors, tracking cellular responses to chemokine, recognition of GAG-bound chemokines, and the production of chemokine receptor complexes for structural and biophysical studies INDICE: 1. Chemokine detection using receptors immobilized on the surface of Surface Plasmon Resonance sensors Mario Mellado2. Tracking Cellular Responses to Chemokine with Microfluidic Devices Ann Richmond3. Generating chemokine analogues with enhanced pharmacological properties using phage display Oliver Hartley4. Methods for the recognition of GAG-bound chemokines Amanda Proudfoot and Pauline Bonvin 5. Monitoring scavenging activity of chemokine receptors Marcus Thelen and Barbara Moepps 6. Molecular Imaging Assays for Chemokine Receptor Signaling Gary Luker7. Analysis of arrestin recruitment to chemokine receptors by Bioluminescence Resonance Energy Transfer Nikolaus Heveker8. Probing biased signaling in chemokine receptors Mette Marie Rosenkilde9. Fluorescent chemokines as cell sorting agents Gerry Graham10. Mutagenesis by phage display Amanda Proudfoot and Pauline Bonvin 11. Studying chemokine function in a murine model of inflammatory arthritis Andrew Luster12. Production of chemokine receptor complexes for structural and biophysical studies Martin Gustavsson and Tracy M. Handel 13. In vivo proof of concept models to study leukocyte migration induced by chemokines Mauro Martins Teixeira14. Monitoring chemokine receptor trafficking by confocal immunofluorescence microscopy Adriano Marchese15. Migration assays for visualizing the patterning of chemokine gradients by an atypical chemokine receptor Reinhold Förster16. Role of chemokine/glycosaminoglycan interaction in chemokine mediated leukocyte migration In vitro and in vivo John Kirby and Simi Ali 17. Examining Roles of Glycans in Chemokine-Mediated Dendritic-Endothelial Cell Interactions Mark Fuster18. Preparation and analysis of N-terminal chemokine receptor peptides containing sulfotyrosines Chris Veldkamp19. Cysteine trapping for modeling and structure determination of receptor:chemokine complexes Irina Kufareva and Tracy M. Handel 20. Analysis of G protein and b-arrestin activation in chemokine receptor signaling Massimo Locati and Elena Borroni 21. Analysis of chemokine receptor expression on leukocyte subpopulations by flow cytofluorimetric techniques Massimo Locati and Raffaella Bonecchi 22. Chemokine receptors: from binding to signaling Martine Smit
- ISBN: 978-0-12-802171-2
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Fecha Publicación: 01/03/2016
- Nº Volúmenes: 1
- Idioma: Inglés