While much information has been gained over the last two decades regarding the molecular nature of prion proteins, only recently have several research groups begun to cast light on turning the normal prion protein into its aberrant, infectious form. In Prion Protein Protocols, an international team of experts provide an up-to-date collection of current methods in this unique area of neuroscience. The chapters contained in this volume, both protocols and useful review chapters, feature topics such as the cell biology of prions, techniques and approaches to studying prion infection in cultured cells, how these systemscan be used as a rapid bioassay, and prion protein misfolding. As a volume inthe highly successful Methods in Molecular Biology™ series, the chapters include readily reproducible, step-by-step laboratory protocols, lists of the necessary materials and reagents, and the Notes section, which provides tips on troubleshooting and avoiding known pitfalls. Most current collection of protocols in prion disease research Fully described methods chapters from leaders in the field with easy to follow instructions and notes sections Provides methods for studying prion infection in cell cultures with a review chapter on currentcell models Detailed protocols for prion protein conversion assays using current technologies with a review highlighting their use INDICE: From the contents Cell culture models to unravel prion protein function and aberrancies in prion diseases.- Investigation of PrPC metabolism andfunction in live cells: methods for studying individual cells and cell populations.- Immunodetection of PrPSc using Western and slot blotting techniques.- Assaying Prions in Cell Culture: The Standard Scrapie Cell Assay (SSCA) and the Scrapie Cell Assay in End Point Format (SCEPA).- Generation of cell lines propagating infectious prions and the isolation and characterization of cell derived exosomes.- Neurotoxicity of prion peptides on cultured cerebellar neurons.- Understanding the nature of prion diseases using cell-free assays.- Methodsfor Conversion of Prion Protein into Amyloid Fibrils.- Amplification of Purified Prions In Vitro.- Expression and Purification of Full-Length Recombinant PrP of High Purity.- Analysis of PrP conformation using circular dichroism.
- ISBN: 978-1-58829-897-3
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 345
- Fecha Publicación: 01/07/2008
- Nº Volúmenes: 1
- Idioma: Inglés