Ubiquitination and Protein Stability - Part B Volume 620, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapter written by an international board of authors. Topics of note in this new release include the Preparation of ubiquitinated nucleosomes with native and non-hydrolyzable linkages, Methods to measure ubiquitin chain length and linkage, Genetic approaches to study the yeast ubiquitin system, Enzymatic preparation of monoubiquitinated proteins, Methods to distinguish the function of ubiquitin in autophagy and the proteasome pathway, the Purification and characterization of enzyme activity of USPs, and much more. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in this series on enzymology Updated release includes the latest information on methods to measure ubiquitin chain length and linkage, genetic approaches to study the yeast ubiquitin system, amongst many other timely topics INDICE: Assays of SUMO protease function in mammalian cellsStefan MüllerIn vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylationByung-Hoon Lee Methods to study proteasome regulatory particle assemblyRobb TomkoNative mass spectrometry approaches to study the proteasomeMichal SharonSingle-molecule methods to study the ubiquitin-proteasome systemYing Lu Assays for the function of ubiquitin in the mammalian endocytic pathwaySimona Polo Measuring parkin and ubiquitin function in mitophagyKeiji Tanaka Assays for protein retrotranslocation in ERADRandy HamptonAssays for protein quality control at the inner nuclear membraneChristian Schlieker LGG-1 western blotting as a read-out for autophagy in C. elegansThorsten Hoppe Dissecting the elementary enzymatic activities of Ubc7, a key E2 conjugating enzyme of the ERAD pathway Tommer Ravid Methods to study phosphoribosylated ubiquitin ligation and removalZhao-Qing LuoAssays for ubiquitin-like protein ligation and proteasome function in archaeaJulie Maupin-FurlowUbiquitin and Related Protein Modifier SystemsHuib Ovaa
- ISBN: 978-0-12-818667-1
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 15/04/2019
- Nº Volúmenes: 1
- Idioma: Inglés