Biophysical Characterization of Proteins in Developing Biopharmaceuticals

Biophysical Characterization of Proteins in Developing Biopharmaceuticals is concerned with the analysis and characterization of the higher-order structure (HOS) or conformation of protein based drugs. Starting from the very basics of protein structure this book takes the reader on a journey on how to best achieve this goal using the key relevant and practical methods commonly employed in the biopharmaceutical industry today as well as up and coming promising methods that are now gaining increasing attention. As a general resource guide this book has been written with the intent to help today's industrial scientists working in the biopharmaceutical industry or the scientists of tomorrow who are planning a career in this industry on how to successfully implement these biophysical methodologies. In so doing a keen focus is placed on understanding the capability of these methodologies in terms of what information they can deliver. Aspects of how to best acquire this biophysical information on these very complex drug molecules, while avoiding potential pitfalls, in order to make concise, well informed productive decisions about their development are key points that are also covered. Presents the reader with a clear understanding of the real world issues and challenges in using these methods.Highlights the capabilities and limitations of each method.Discusses how to best analyze the data generated from these methods.Points out what one needs to look for to avoid making faulty conclusions and mistakes.In total it provides a check list or road map that empowers the industrial scientists as to what they need to be concerned with in order to effectively do their part in successfully developing these new drugs in an efficient and cost effective manner. INDICE: 1. The complexity of protein structure and the challenges in developing biopharmaceuticals (Steven A. Berkowitz, and Damian Houde) 2. The information obtainable and justification for biophysical analysis of biopharmaceuticals (Damian Houde, and Steven A. Berkowitz) 3. Biophysical tools currently used in the biopharmaceutical industry (and prospective tools) (Damian Houde, and Steven A. Berkowitz) 4. What type of information do we need from each biophysical tool? (Damian Houde, and Steven A. Berkowitz) 5. 5 The value of UV, fluorescence and FTIR spectroscopy in biopharmaceutical development (Mark Brader) 6. Circular dichroism spectroscopy for protein characterisation: pharmaceutical applications (Andrew J. Miles, and B.A. Wallace) 7. The use of size-exclusion chromatograph (SEC) in biopharmaceutical process development (Steven A. Berkowitz) 8. Characterization of proteins in solution using small-angle X-ray solution scattering (Lee Makowski) 9. The role of analytical ultracentrifugation (AUC) in biopharmaceutical process development (John Philo) 10. Subvisible and visible particle analysis in biopharmaceutical research and development (Andrea Hawe, Sarah Zoells, Angelika Freitag, and John Carpenter) 11. Differential scanning calorimetry in the biopharmaceutical sciences (Stephen J. Demarest, and Verna Frasca) 12. Tackling new biological problems with solution-phase hydrogen/deuterium exchange monitored by mass spectrometry (George Bou-Assaf, Damian Houde, and Alan G. Damian Marshall) 13. One- and two-dimensional NMR techniques (Daron I. Freedberg, Yves Aubin, and David Keire) 14. Present status of biophysical measurements in biopharmaceutical process development (Damian Houde, and  Steven A. Berkowitz)  

• ISBN: 978-0-444-59573-7
• Editorial: Elsevier
• Encuadernacion: Cartoné
• Páginas: 366
• Fecha Publicación: 01/09/2014
• Nº Volúmenes: 1
• Idioma: Inglés