Techniques for Biochemical Analysis

Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested protocols, outlining the principles upon which each technique is founded, as well as providing instructions on equipment setup and use, buffer preparation, reagents required, safety considerations and analysis of findings. Beginning with an introduction to biochemistry and laboratory procedures, the book moves on to specific methods focused on investigation of carbohydrates, proteins, enzymes, plant hormones, minerals, amino acids, and more. The large range of protocols covered in this foundational, how-to reference are interdisciplinary and adaptable to a variety of areas, making this an ideal resource for researchers across various fields, including biochemistry, molecular biology, medical sciences, plant physiology, agriculture, and related subjects. Features step-by-step methods for biochemical analysis of a variety of compoundsExplores methods that are applicable and adaptable across a variety of fields, including biochemistry, molecular biology, and related areasProvides detailed instructions on how to prepare buffers, the equipment to be used, and the analysis of a variety of molecules, including carbohydrates, lipids, proteins, and hormonesContains interdisciplinary and adaptable methods and techniques INDICE: 1. Introduction to2. Introduction to laboratory procedures3. Preparation of solutions and buffers4. Qualitative analysis of carbohydrates5. Qualitative analysis of proteins6. pH (negative logarithm of hydrogen ion concentration)7. Spectrophotometry8. Carbohydrates9. Amino acids and proteins10. Enzyme assays11. Vitamin assays12. Phenolics13. Plant pigments14. Plant15. Antinutritional factors in plants16. Toxins17. Extraction and identification of polyacetylenes18. Alkaloids19. Plant hormones20. Immunochemical methods21. Nitrogenase activity22. Chlorophyll23. Proximate analysis24. Minerals25. Antinutritional factors in plants26. Antioxidants27. Lipids

• ISBN: 978-0-443-15914-5
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 256
• Fecha Publicación: 09/04/2024
• Nº Volúmenes: 1
• Idioma: Inglés