Methods in Enzymology: Visualizing RNA Dynamics in the Cell continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods visualizing RNA dynamics in the cell, and includes sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2, and visualization of 5S dynamics in live cells using photostable corn probe. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in visualizing RNA dynamics in the cellContains sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2 and visualization of 5S dynamics in live cells using photostable corn probe INDICE: 1. Visualization of 5S dynamics in live cells using photostable Corn probe Samie R, Jaffrey2. Fluctuation analysis to measure dynamics of nascent RNA Daniel R. Larson3. Imaging the first round of translation Jeffrey A. Chao4. Identification of RNA cis-regulatory sequences Elizabeth J. Grayhack5. IRAS: high-throughput identification of novel alternative splicing regulators Sika Zheng6. Analysis of alternative splicing of cassette exons at single-cell level Konstantin A. Lukyanov7. Analysis of nonsense-mediated mRNA decay at the single cell level Konstantin A. Lukyanov8. Imaging native bacterial RNA in live cells Natalia E. Broude9. IMAGEtags: quantifying mRNA transcription in real time with multiaptamer reporters Marit Nilsen-Hamilton and Judhajet Ray 10. Imaging endogenous mRNA in mammalian cells using fluorescent protein-tagged Pumilio Takeaki Ozawa11. Single RNA sensitive probes for characterization of virus dynamics in cells Philip J. Santangelo12. Applications of Hairpin DNA Functionalized Gold Nanoparticles for Imaging mRNA in Living Cells Ashwath Jayagopal and Diana Bratu 13. Imaging Single mRNA Dynamics in Live Neurons and Brains Hye Yoon Park14. A method for expressing abundant, stable, circular RNAs in vivo using tRNA splicing A. Gregory Matera15. MERFISH: spatially resolved, highly multiplexed RNA profiling in single cells Jeffrey R. Moffitt16. Plant RNA labeling using MS2 Manfred Heinlein
- ISBN: 978-0-12-802292-4
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 514
- Fecha Publicación: 01/06/2016
- Nº Volúmenes: 1
- Idioma: Inglés