In the years since the release of the popular first edition, the field of High Throughput Screening (HTS) has evolved considerably, from a small niche areaof study to a major, essential scientific technique. In High Throughput Screening: Methods and Protocols, Second Edition, experts in the field fully updatethe previous edition to best serve as an introduction to HTS for scientists who are just entering the field, and as a provider of key, significant detail so as to be useful to scientists in established HTS operations. Featuring new screening technologies as well as many well established methods, chapters offercomprehensive treatment of the activities directly related to HTS, such as compound library management, data handling, and robotics. Presents a comprehensive overview of screening techniques to create a How-to book for HTS Contains detailed protocols for common HTS methods Details protocols and techniques for cellular and biochemical assays Provides everything one needs to know in orderto understand the most common HTS detection technologies Serves as an essential tool and process guide for experienced HTS professionals and beginners alike INDICE: Design and Implementation of High Throughput Screening Assays.- Creation of a Small High-Throughput Screening Facility.- Informatics in CompoundLibrary Management.- Statistics and Decision Making In High Throughput Screening.- Enzyme Assay Design for High Throughput Screening.- Application of Fluorescence Polarization in HTS Assays.- Screening G-Protein Coupled Receptors: Measurement of Intracellular Calcium Using the Fluorometric Imaging Plate Reader.- High Throughput Automated Confocal Microscopy Imaging Screen of a Kinase Focused Library to Identify p38 Mitogen Activated Protein Kinase Inhibitors Using the GE InCell 3000 Analyzer.- Recent Advances in Electrophysiology Based Screening Technology and the Impact upon Ion Channel Discovery Research.- Automated Patch Clamping Using the QPatch.- High Throughput Screening of the Cyclic AMP-Dependent Protein Kinase (PKA) Using the Caliper Microfluidic Platform.- Use of Primary Human Cells in High Throughput Screens.
- ISBN: 978-1-60327-257-5
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 265
- Fecha Publicación: 01/08/2009
- Nº Volúmenes: 1
- Idioma: Inglés