Pseudokinases, Volume 667, the latest release in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters, including the Production and Purification of the PEAK pseudokinases for structural and functional studies, Structural biology and biophysical characterization of Tribbles pseudokinases, Detecting endogenous TRIB protein expression and its downstream signaling, Analysis of human Tribbles 2 pseudokinase, Expression, purification and examination of ligand-binding to IRAK pseudokinases, Characterization of pseudokinase ILK-mediated actin assembly, Biochemical examination of Titin pseudokinase, Approaches to study pseudokinase conformations, CRISPR editing cell lines for reconstitution studies of pseudokinase function, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology serials Includes the latest information on Pseudokinases INDICE: 1. Production and Purification of the PEAK pseudokinases for structural and functional studies2. Structural biology and biophysical characterisation of Tribbles pseudokinases3. Detecting endogenous TRIB protein expression and its downstream signalling4. Analysis of human Tribbles 2 pseudokinase5. Expression, purification and examination of ligand-binding to IRAK pseudokinases6. Characterization of pseudokinase ILK-mediated actin assembly7. Biochemical examination of Titin pseudokinase8. Approaches to study pseudokinase conformations9. CRISPR editing cell lines for reconstitution studies of pseudokinase function10. Proximity ligation approaches to define the interactomes of pseudokinases11. Determining the role of the pseudokinase domain in guanylyl cyclase A and B signaling12. Strategies to study and stimulate bacterial pseudokinases13. Dynamics of Kinases and Pseudokinases by HDX-MS14. In-cell thermal shift assay for drug binding analysis to receptor pseudokinases15. Pseudoenzymology of Kinase Suppressor of Ras16. Computational and evolutionary insights into pseudokinase mechanisms17. Methods to identify small molecule allosteric modulators of the STRAD pseudokinase18. Pharmacological targeting of the pseudokinase HER319. Considerations and analysis of the unusual pseudokinase BUBR120. The pseudokinase domain of receptor guanylyl cyclases: binding of ATP and allosteric regulation of guanylyl cyclase activity21. Methods for discovering catalytic activities for pseudokinases22. Efficient Expression, Purification, and Visualization by Cryo-EM of Fully-Glycosylated Unliganded HER323. An effective strategy for ligand-mediated pulldown of the HER2/HER3 receptor complex and cryo-EM structure determination at low sample concentrations24. Identification of small molecule binders for pseudokinases25. Identification and characterization of Tyk2-JH2 pseudokinase domain inhibitors26. Monitoring the ATPase activity of Bud32 and its role in tRNA binding
- ISBN: 978-0-323-91541-0
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 15/05/2022
- Nº Volúmenes: 1
- Idioma: Inglés