Biological microarrays: methods and protocols

Recent developments in microarray technology have changed the landscape of biology and biomedical research, and they have revolutionized RNA and DNA research. In Biological Microarrays: Methods and Protocols, expert researchers explore exciting new developments in the field, providing a comprehensive approach to biological microarrays that conveys not only the state-of-the-art fundamentals, but also includes applications of the most innovative methods. Chapters address both the application of biological microarrays, including DNA/RNA, apatmer, proteins, tissues, oligonucleotides, carbohydrates, biomaterials, cells, bacteria, and virus microarrays, and also explore the different techniques used for generating microarray platforms. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes sectionwhich shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and revolutionary, Biological Microarrays: Methods and Protocols serves as aprimary source for academics, practitioners, and professionals in related fields, including biologists, biotechnologists, biochemists, analytical chemists,and biomedical, physical, and microsystems engineers, to name a few, appealing to all of those interested in the present and future state of biological microarray research. Comprehensive presentation of various biological microarraysand their applications -Includes methods for analysis and characterization ofthe resulting arrays -Provides description of synthesis methods and areas of use INDICE: RNA and DNA Microarrays.- Aptamer Arrays.- Oligonucleotide microarrays for identification of microbial pathogens and detection of their virulence-associated or drug-resistance determinants.- Protein MicroArrays printed from DNA MicroArrays.- Lithographically-defined two- and three-dimensional tissuemicroarrays.- Ratiometric lectin microarray analysis of the mammalian cell surface glycome.- Cell microarrays based on hydrogel microstructures for the application to cell-based biosensor.- Fabrication of Bacteria and Virus Microarrays Based on Polymeric Capillary Force Lithography.- 3D Polymer Scaffold Arrays.- PDMS microfluidic capillary systems for patterning proteins on surfaces andperforming miniaturized immunoassays.- Merging Photolithography and Robotic Protein Printing to Create Cellular Microarrays.- Generation of Protein and Cell Microarrays on Functionalized Surfaces.- Microprinting of Liver Micro-organ for Drug Metabolism Study.- Microcontact printing.- Micromolding for the Fabrication of Biological Microarrays.- Progress Report on Microstructured SurfacesBased on Chemical Vapor Deposition.- Methods for Forming Human Microvascular Tubes in Vitro and Measuring Their Macromolecular Permeability.- Microarray Bioinformatics.

• ISBN: 978-1-934115-95-4
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 304
• Fecha Publicación: 29/10/2010
• Nº Volúmenes: 1
• Idioma: Inglés