Fluorescence Microscopy: From Principles to Biological Applications

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super–resolution and single–molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years. INDICE: Introduction . Principles of Light Microscopy . Fluorescence Microscopy . Fluorescence Labeling . Confocal Microscopy . Fluorescence Photobleaching and Photoactivation Techniques . Förster Resonance Energy Transfer and Fluorescence Lifetime Imaging . Multi–Photon Excitation . Single Molecule Microscopy in the Life Sciences . Super–Resolution Microscopy: Interference and Pattern Techniques . STED Microscopy . Light Sheet Fluorescence Microscopy . Appendix A: Practical Guide to Optical Alignment . Appendix B: Matrices and Images.

• ISBN: 978-3-527-33837-5
• Editorial: Wiley VCH
• Encuadernacion: Cartoné
• Páginas: 504
• Fecha Publicación: 19/04/2017
• Nº Volúmenes: 1
• Idioma: Inglés