Despite its inherent controversy, the exploration of the human embryo can unlock many of the answers to our deepest biological questions. In Human Embryogenesis: Methods and Protocols, internationally recognized researchers contribute detailed methods to analyze various aspects of the embryogenesis process. While comprehensively covering subjects such as the molecular mechanisms of embryonic development, in vitro fertilization, cloning, and the laws and ethical considerations of working with embryos, the volume also addresses critical features of fetal and placental development as well as of uterine biology. Writtenin the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. An easily accessiblereference book, written by internationally recognized researchers Covers a wide range of methods and protocols, from molecular mechanisms to cell signalingto ethical considerations, which aid the exploration of human embryogenesis INDICE: From the contents Human Embryogenesis: Overview.- Differential Gene Expression in the Uterus and Blastocyst During the Reactivation of Embryo Development in a Model of Delayed Implantation.- Coculture of Decidua and Trophoblast to Study Proliferation and Invasion.- Isolation and Culture of Term Human Cytotrophoblast Cells and In Vitro Methods for Studying Human Cytotrophoblast Cells Calcium Uptake.- Endocrinology and Cell Signaling in Human Villous Trophoblast.- Gestation-Induced Uterine Vascular Remodeling.- Placental and FetalSteriodogenesis.- Current Methods in Investigating the Development of the Female Reproductive System.- A Revised and Improved Method for the Isolation of Seminiferous Tubule-Enriched Fractions that Preserves the Phosphorylated and Glycosylated Forms of Proteins.- A Novel Technical Approach for the Measurement of Individual ACAT-1 and ACAT-2 Enzymatic Activity in the Testis.
- ISBN: 978-1-60327-008-3
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 275
- Fecha Publicación: 01/06/2009
- Nº Volúmenes: 1
- Idioma: Inglés