Biomolecule analysis and manipulation: methods and protocols

In recent years, large-scale advances in technology have led to greater understanding of the world on a much tinier scale: the biomolecular level. In Biomolecule Analysis and Manipulation: Methods and Protocols, expert researchers from across the globe explore the technology which makes this analysis possible,investigating the worlds of microfluidics and nanotechnologies, and examiningphysical science techniques for the separation, detection, manipulation, and analysis of biomolecules. This volume contains innovative protocols on the application of microfluidics and the utilization of physical science-related technologies that will prove to be invaluable in the field of molecular biology. Chapters contain cutting edge applications of emerging nanotechnologies, including quantum dots and molecular fluorescence for the imaging and tracking of biomolecules. Provides current information on developments in nanotechnologies and their use in biomolecular delivery, gene therapy and expression control Considers the applications of microfluidics and nanopores in the separation, manipulation, detection, and analysis of biomolecules Presents emerging technologies, including applications of quantum dots and molecular fluorescence in the tracking and imaging of biomolecules INDICE: From the contents HPLC-Chip/MS technology in proteomic profiling.-Nanofluidic channels fabrication and manipulation of DNA molecules.- A single-molecule barcoding system using nanoslits for DNA analysis—Nanocoding.- Microfluidic Devices with Photodefinable Pseudo-valves for Protein Separation.- Microfluidic chip designed for measuring biomolecules through microbead-based quantum dot fluorescence assay.- DNA Focusing Using Microfabricated Electrode Arrays.- Solid-state Nanopore for Detecting Individual Biopolymers.- Inserting and manipulating DNA in a nanopore with optical tweezers.- Forming an a-hemolysin nanopore for single molecule analysis.- Nanopore Force Spectroscopy on DNA Duplexes.- Quantitative chemical analysis of single cells.- Trapping and Detection of Single Molecules in Water.- ZnO Nanorods as an Intracellular Sensor forpH Measurements.

• ISBN: 978-1-934115-40-4
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 595
• Fecha Publicación: 01/05/2009
• Nº Volúmenes: 1
• Idioma: Inglés