Modern Methods of Drug Design and Development, a volume in the Methods in Enzymology series highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Recombinant protein purification for structural and kinetic studies, Steady-state kinetic analysis of reversible enzyme inhibitors, Steady-State Enzyme Kinetics, Analysis of enzyme kinetic data using ICEKAT, NMR techniques in drug discovery, Dynamic simulations and pre-steady state kinetics to guide drug discovery, Design and assay of substrate-product analogues for racemases and epimerases, Sensitive high throughput methods to screen for P450 inhibition: A- MI complex forming drugs, and more. Other chapters cover Sensitive high throughput methods to screen for P450 inhibition: B-Heme loss causing drugs, Discovery and development of inhibitors of acetyltransferase Eis to combat Mycobacterium tuberculosis, Crystallographic fragment screening in academic cancer drug discovery, Fast fragment- and compound-screening pipeline at the Swiss Light Source, Chemical biology, enzymology and drug discovery, PROTACs, Proximity-Induced Pharmacology (PROTACs), and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Updated release includes the latest information on Modern Methods of Drug Design and Development INDICE: 1. Recombinant protein purification for structural and kinetic studiesMatthew Lloyd, Otsile Mojanaga and K Ravi Acharya2. Steady-state kinetic analysis of reversible enzyme inhibitorsMatthew Lloyd3. Steady-State Enzyme KineticsBharath Srinivasan4. Analysis of enzyme kinetic data using ICEKATBrian C. Smith5. NMR techniques in drug discoveryTim Woodman6. Dynamic simulations and pre-steady state kinetics to guide drug discoveryKen Johnson7. Design and assay of substrate-product analogues for racemases and epimerasesStephen L. Bearne8. Sensitive high throughput methods to screen for P450 inhibition: A- MI complex forming drugsRheem Totah9. Sensitive high throughput methods to screen for P450 inhibition: B-Heme loss causing drugsRheem Totah10. Discovery and development of inhibitors of acetyltransferase Eis to combat Mycobacterium tuberculosisSylvie Garneau-Tsodikova and Oleg Tsodikov11. Crystallographic fragment screening in academic cancer drug discoveryJane Endicott, Martin Noble and Mathew Martin12. Fast fragment- and compound-screening pipeline at the Swiss Light SourceJustyna A. Wojdyla, May E. Sharpe and Guenter Fritz13. Chemical biology, enzymology and drug discoveryKevin Dalby14. PROTACsMatthias Baud15. Proximity-Induced Pharmacology (PROTACs)Matthieu Schapira16. Chemo-enzymatic Labelling with asparaginyl endopeptidase (AEP)Louis Luk17. Development of 5-Aminolevulinic Acid Prodrugs for Photodynamic Therapy and PhotodiagnosisIan Eggleston18. Systems biology and single cell approachesUdo Opperman
- ISBN: 978-0-443-15871-1
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 598
- Fecha Publicación: 17/10/2023
- Nº Volúmenes: 1
- Idioma: Inglés