Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocolsIn this volume we have brought together a number of core protocols concentrating on RNA INDICE: Computational Prediction of RNA Secondary Structure Reverse-transcription PCR (RT-PCR) Northern blotting Explanatory Chapter: Nuclease Protection Assays In Vitro Transcription from Plasmid or PCR-amplified DNA Measurement of In Vivo RNA Synthesis Rates Method for measuring mRNA decay rate in Saccharomyces cerevisiae Analysis of polysomes from bacteria Polysome Profile Analysis - Yeast Polysome analysis of mammalian cells Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3'; ends, and poly(A) tails of RNA RNA Radiolabeling Fluorescently Labeling Synthetic RNAs Analysis of RNA by analytical polyacrylamide gel electrophoresis RNA purification by preparative polyacrylamide gel electrophoresis Explanatory Chapter: Nucleic Acid Concentration Determination RNA purification - precipitation methods Reverse Transcriptase Dideoxy Sequencing of RNA RNA Structure Experimental Analysis - Chemical Modification Structural Analysis of RNA Backbone Using In-Line Probing
- ISBN: 978-0-12-420037-1
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 472
- Fecha Publicación: 01/11/2013
- Nº Volúmenes: 1
- Idioma: Inglés