Mitosis and Meiosis, Part A, Volume 144, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Analyzing the Spindle Assembly Checkpoint in human cell culture, an Analysis of CIN, a Functional analysis of the tubulin code in mitosis, Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis, Applying the auxin-inducible degradation (AID) system for rapid protein depletion in mammalian cells, Small Molecule Tools in Mitosis Research, Optogenetic control of mitosis with photocaged chemical, and more. Contains contributions from experts in the field from across the worldCovers a wide array of topics on both mitosis and meiosisIncludes relevant, analysis based topics INDICE: 1. Analysing the Spindle Assembly Checkpoint in human cell culture Jonathon Pines 2. Analysis of CIN Duane Compton 3. Functional analysis of the tubulin code in mitosis Helder Maiato 4. Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis Kara L. McKinley 5. Applying the auxin-inducible degradation (AID) system for rapid protein depletion in mammalian cells Andrew Jon Holland 6. Small Molecule Tools in Mitosis Research Thomas U. Mayer 7. Optogenetic control of mitosis with photocaged chemical dimerizers Michael Lampson 8. Measuring forces in living cells Tom Maresca 9. Employing the One-Cell C. elegans Embryo to Study Cell Division Processes Arshad Desai, Neil Hattersley and Pablo Lara-Gonzalez 10. A cell-free system of Drosophila egg explants supporting native mitotic cycles Ivo Andreas Telley 11. Living Xenopus oocytes, eggs and embryos as models for cell division Bill Bement 12. Chromosome spreads in somatic cells and oocytes OR TIRF to study cohesin Jan-Michael Peters 13. In Vitro Reconstitution of Microtubule Lateral to End-on Conversion Assisted by the Kinetochore Kinesin CENP-E Ekaterina L. Grishchuk and Manas Chakraborty 14. Isolation of mitotic chromosomes from vertebrate cells and characterisation of their proteome by mass spectrometry William C. Earnshaw and Itaru Samejima 15. Isolation of yeast kinetochores Sue Biggins 16. Characterization of DNA helicases and nucleases from meiotic extracts of S. cerevisiae. Joao Matos 17. Single-nucleus Hi-C in oocytes and zygotes Kikue Tachibana-Konwalski, Johanna Gassler and Ilya Flyamer 18. Induction of Fetal Primary Oocytes and the Meiotic Prophase from Mouse Pluripotent Stem Cells Mitinori Saitou 19. Electrical-assisted microinjection for analysis of fertilisation and cell division in mammalian oocytes and early embryos Greg FitzHarris and Karl Swann 20. Single cell genomics to study DNA and chromosome changes in human gametes and embryos Eva R. Hoffmann 21. Cytoplasmic removal, enucleation, and cell fusion of mouse oocytes Tomoya Kitajima
- ISBN: 978-0-12-814144-1
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 362
- Fecha Publicación: 01/06/2018
- Nº Volúmenes: 1
- Idioma: Inglés