Enzymes of Epigenetics: Part B, one of two new volumes in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods that are employed in the study of epigenetic regulation, including structural, biochemical, molecular, biological, cellular, computational, and systems approaches. Topics include chromatin structure and histones, posttranslational histone modification enzymes and complexes, histone modification binders, DNA modifications and nucleic acid regulators, epigenetic technologies, and small molecule epigenetic regulators and biological connections. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Contains two new volumes that cover research methods in enzymes of epigeneticsCovers such topics as chromatin structure and histones, posttranslational histone modification enzymes and complexes, histone modification binders, DNA modifications and nucleic acid regulators, epigenetic technologies and small molecule epigenetic regulators, and biological connections INDICE: Chromatin Structure and Histones 1. Analysis of euchromatin and heterochromatin assembly 2. Analysis of telomeres and biochemical and structural analysis of associated proteins 3. Preparation and biophysical characterization of centromeric nucleosomes and associated proteins 4. Functional characterization of histone chaperones 5. Chromatin Assembly-Strategies and Quality Control 6. Preparation, crystallization and structure determination of chromatin enzyme/nucleosome complexes 7. Fluorescence based methods for characterizing ATP-dependent chromatin remodeling enzymes Posttranslational Histone Modification Enzymes and Complexes 8. Preparation, biochemical analysis and structure determination of SET protein histone methyltransferases 9. Expression, purification and biochemical analysis of the LSD1/KDM1A histone demethylase 10. Purification, biochemical analysis, and structure determination of JmjC lysine demethylases 11. Low- and high-throughput assays for histone acetyltransferases 12. Preparation and analysis of chromatin modifying complexes 13. Preparation and biochemical analysis of classical histone deacetylases 14. Preparation, biochemical analysis and structure determination of sirtuin histone deactylases Histone Modification Binders (Readers) 15. Preparation, biochemical analysis and structure determination of bromodomains 16. Preparation, biochemical analysis and structure determination of methyllysine recognition domains DNA Modifications and Nucleic Acid Regulators 17. Preparation, biochemical analysis and structure determination of DNA methylation enzymes 18. Preparation of TET enzymes and characterization of 5-methyl-cytosine oxidation 19. Detection and analysis of long non-coding RNAs Epigenetic Technologies 20. Histone posttranslational modifications detected by mass spectrometry 21. Chemical approaches to study histone modifications 22. Chemical synthesis and application of mechanism-based inhibitors of LSD1 histone demethylases 23. Global active-site profiling of endogenous cellular lysine acetyltransferases 24. Quantifying histone PTM turnover with metabolic labeling 25. Substrate Specificity Profiling of Histone Modifying Enzymes by Peptide Microarray 26. Analysis of histone dynamics in vivo 27. Chemical biology approaches for characterization of epigenetic regulators in health and disease 28. FISH-IF approach is to study genome organization in fission yeast Small Molecule Epigenetic Regulators 29. Preparation and analysis of class III deacetylase (sirtuin) inhibitors 30. Preparation and analysis of class III deacetylase (sirtuin) activators 31. Preparation and analysis of methyltransferase inhibitors Biological connections 32. Methods to study connections between epigenetics and metabolism 33. Methods to study connections between epigenetics and neurobiology 34. ChIP-seq to map the epigenome of senescent cells 35. Methods to study connections between epigenetics and the immune system 36. ArrayNinja: An Open Source Platform for Unified Planning and Analysis of Microarray Experiments
- ISBN: 978-0-12-805381-2
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 04/07/2016
- Nº Volúmenes: 1
- Idioma: Inglés