HPLC methods for clinical pharmaceutical analysis: a user's guide

Filling a gap in the literature for a hands-on guide focusing on everyday laboratory challenges, this English edition has been expanded and revised using the feedback received on the successful German precursor. Throughout the book, Professor Mascher draws on his 30 years of experience and provides abundant practical advice, troubleshooting and other hints highlighted in boxes, as well as a broad selection of walk-through case studies. Based on a course taught bythe author, the first part of the book intuitively explains all steps of a routine pharmaceutical analysis and explains how to set up a robust, inexpensiveand efficient method for a given substance. In the second part he includes 20worked example cases that highlight common challenges and how to overcome them. With its appendix containing tried-and-tested analytical methods for 100 clinically relevant substances from the author's own laboratory, complete with spectral and MS data as well as literature references, this is a life-long companion for everyone working in clinical, pharmaceutical and biochemical analysis. INDICE: INTRODUCTION Example case #1: Ibuprofen in Plasma Example case #2:Tryptophan in Urine Example case #3: Paclitaxel in Tissue ANALYTICAL STRATEGYLimit of Detection Analyte Structure Analyte Solubility Choice of Detector SAMPLE PREPARATION Dilution Precipitation of Proteins Extraction SEPARATION BY HPLC Pumps and Equipment Degassing Injection HPLC Columns DETECTION Requirements for Pharma/Bioanalysis Requirements for TDM and Other Clinical Applications CHEMICAL DERIVATISATION VALIDATION DIFFICULT SUBSTANCES Dealing with Unstable Analytes Dealing with Decomposition Products ANALYSIS OF METABOLITES USE OF INTERNAL REFERENCES CASE STUDIES Acetyl carnitine in plasma Acetyl cysteine in plasma Acyclovir in plasma and urine Caffeine in plasma Diazepam in plasma Diclofenac in plasma Dihyralazine in plasma Duramycin in plasma Fluticason propionate in plasma Hydroxy triamterene sulfate and triamterene in plasma and urine Ibuprofen in plasma (including separation of enantiomers) Minocyclin in plasmaNorfloxacin in plasma and urine Paclitaxel in plasma, urine, and tissue Paracetamol (acetaminophen) in plasma Pimelic acid in plasma and urine 8-Prenylnaringenin in plasma and in different tissues Silibinin in plasma Valnemulin in plasma, tissues, and animal feed Vitamin B1 (total thiamine) in plasma APPENDIX Short summaries of analysis schemes for approx. 100 clinically relevant substances in a special one-page layout

• ISBN: 978-3-527-33129-1
• Editorial: Wiley-VCH
• Encuadernacion: Cartoné
• Páginas: 264
• Fecha Publicación: 25/01/2012
• Nº Volúmenes: 1
• Idioma: Inglés