With the advent of modern methodology, the phenomenal complexity of the protein components and regulatory steps involved in mitosis has become approachable. In Mitosis: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate the mechanism of mitosis at the molecular level. Ideal for both experienced and novice scientists, the volume includes step-by-step methods covering inactivation of genes of interest, depletion of proteins of interest, biochemical and microscope-based techniques, and procedures to monitor and measure key mitotic processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the respective topics, lists of thenecessary materials and reagents, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Provides an easily accessible reference volume for investigating mitosis put together by leading researchers in the field Presents approaches in both mammalian and chicken cellsystems as well as in model single-cell and multi-cellular organisms Covers abroad range of biochemical, genetic and cell biological methods focused on dissecting specific mitotic processes INDICE: From the contents Knock-In and Knock-Out: The Use of Reverse Genetics in Somatic Cells to Dissect Mitotic Pathways.- Functional Dissection of Mitotic Regulators through Gene Targeting in Human Somatic Cells.- RNAi in Drosophila S2 Cells As A Tool For Studying Cell Cycle Progression.- Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast.- Hydrodynamic Analysis of Human Kinetochore Complexes during Mitosis.- Isolation of Protein Complexes Involved in Mitosis and Cytokinesis from Drosophila Cultured Cells.- Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines.- Electron Tomography of Microtubule End-Morphologies in C. elegans Embryos.- Dissecting Mitosis with Laser Microsurgery and RNAi in Drosophila Cells.- Fluorescence Imaging of the Centrosome Cycle in Mammalian Cells.
- ISBN: 978-1-60327-992-5
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 345
- Fecha Publicación: 01/06/2009
- Nº Volúmenes: 1
- Idioma: Inglés