Dr. Tom Moss assembles the new standard collection of cutting-edge techniquesto identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance,site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2ndEdition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.
- ISBN: 978-1-61737-125-7
- Editorial: Humana
- Encuadernacion: Rústica
- Fecha Publicación: 31/07/2012
- Nº Volúmenes: 1
- Idioma: Inglés