PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists

PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is written with an aim of providing researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. PCR Guru covers the history of PCR and the basic science underlying it and discusses what PCR is on the bench level starting with detailed description and tips on primer design and continuing with what are the standard protocols used to perform PCR. Provides troubleshooting tips for various types of modifications of standard protocolsContains unique Good Practices and Tips? that are indispensable for the beginner and expert alikeFeatures Special Cases? with applications of PCR, optimization, and troubleshootingIncludes detailed appendices with tables, figures, and key protocolsOrganized as a systematic, concentrated resource to save time when addressing a PCR problem INDICE: 1. Introduction2. Procedure3. Good PCR Practices4. Troubleshooting5. Tips6. Special CasesAppendix A. Agarose Gel Electrophoresis ProtocolAppendix B. Silver Staining of DNA GelsAppendix C. Plasmid Miniprep ProtocolAppendix D. Restriction Enzyme Digestion ProtocolAppendix E. Protocol for DNA Concentration Estimation by using Hoechst 33258Appendix F. FormulasAppendix G. Primers for Common Promoters, Housekeeping Genes, Reporters, etc.Appendix H. TagsAppendix I. Thermostable DNA polymerasesAppendix J. Example of a Daily PCR LogAppendix K. Common Buffers and SolutionsAppendix L. Useful numbersAppendix M. Yeast colony PCR

• ISBN: 978-0-12-804231-1
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 112
• Fecha Publicación: 01/12/2016
• Nº Volúmenes: 1
• Idioma: Inglés