Next Generation Sequencing demystifies the process of sequencing with a hands-on approach. Offering a theoretical and practical discussion of the how and why behind the kit culture, this publication instructs readers on the science behind the technology and the analysis and application of data. The primer includes a needed pros-and-cons list, protocols, and a discussion of implementing applications. Filling the gap in next generation sequencing literature, Next Generation Sequencing: A Primer provides needed instruction for early researchers, students and researchers outside of their expertise across the biological and biomedical sciences. Provides information on how technology worksDiscusses how and why to prepare samplesInstructs on how to analyze dataCovers basic techniques and history behind the kit culture INDICE: Introduction Current next generation technologies, clonal approaches, single molecule approaches Rationale: NGS enables new biological questions Growth of cells for NGS (using yeast and cultured mammalian cells) Nucleic acid extraction: DNA and RNA PCR, gel electrophoresis and quantitative PCR Overview of diverse types of NGS sequencing libraries. Preparation of genomic DNA and RNA-seq libraries Sequencer Operation: overview of diverse platforms Sequencing on the MiSeq Data extraction and analysis I: quality filters, trimming, demultiplexing and mapping Data analysis II: genome viariants- SNPs, CNVs and rearrangements Data analysis III: RNA seq analysis- gene expression, non-coding sequences, splicing Data analysis IV: Data visualization and data sharing Summary and report preparation Applications of NGS Perspectives: NEXT next generation technologies
- ISBN: 978-0-12-407770-6
- Editorial: Academic Press
- Encuadernacion: Rústica
- Páginas: 240
- Fecha Publicación: 13/10/2014
- Nº Volúmenes: 1
- Idioma: Inglés