With the development of polypeptide ionization processes for mass spectrometry and the rapid processing of large-scale datasets with bioinformatics, researchers now have the tools necessary to propel neuroproteomics research to new levels. In Neuroproteomics: Methods and Protocols, experts in the neurological and analytical sciences present experimental details for applying proteomics to the study of the central nervous system (CNS) and its dysfunction through trauma and disease. Divided into four convenient sections, this collection covers CNS animal models used for neuroproteomics research, methods for separating and analyzing discrete subcomponents of the neuroproteome, large-scale approaches for CNS proteome characterization and quantification, as well as methods that evaluate biofluids and translate neuroproteomic results into clinical platforms. Presents a modular approach to experimental design, covering neurodegenerative models, subproteome selection technologies, quantitative and qualitative mass spectrometry, data analysis and clinical translation of results Detailed guide to developing, running and analyzing broadly applicable neuroproteomic experiments INDICE: From thecontents The Methodology of Neuroproteomics.- Modeling Cerebral Ischemia in Neuroproteomics.- Clinical and Model Research of Neurotrauma.- Neuroproteomic Methods in Spinal Cord Injury.- Modeling Substance Abuse forApplications in Proteomics.- Protein Aggregate Characterization in Models of Neurodegenerative Disease.- Sub-Proteome Processing: Isolation of NeuromelaninGranules from the Human Brain.- Proteomic Analysis of Protein Phosphorylationand Ubiquitination in Alzheimer’s Disease.- Proteomics Identification of Carbonylated and HNE-Bound Brain Proteins in Alzheimer’s Disease.- Mass Spectrometric Identification of in vivo Nitrotyrosine Sites in the Human Pituitary TumorProteome.- Improved Enrichment and Proteomic Analysis of Brain Proteins with Signaling Function by Heparin Chromatography.- Calmodulin Binding Proteome in the Brain.- Separation of the Neuroproteome by Ion Exchange Chromatography.
- ISBN: 978-1-934115-84-8
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 320
- Fecha Publicación: 01/08/2009
- Nº Volúmenes: 1
- Idioma: Inglés