This new volume of Methods in Cell Biology looks at methods for analyzing of biophysical methods in cell biology. Chapters cover such topics as AFM, traction force microscopy, digital holographic microscopy, single molecule imaging, video force microscopy and 3D multicolor super-resolution screening Covers sections on model systems and functional studies, imaging-based approaches and emerging studiesChapters are written by experts in the fieldCutting-edge material INDICE: 1. Cell rheology with microplates Atef Asnacios2. Extracting cell mechanics from quantitative monitoring of cell shape changes Ewa Paluch3. AFM on animal cells Kristian Franze4. Refractive index measurements with digital holographic microscopy Jochen Guck5. Traction force microscopy Manuel Thery6. Advances in Method to Micropattern Traction Force Substrates TBC7. Micropillars for force measurements Benoit Ladoux8. Force measurements during collective cell migration Xavier Trepat9. 3D traction force microscopy to monitor cancer spheroid contractions Timo Betz10. Dissecting spindle architecture by laser ablation Jan Brugues11. Speckle for actin dynamics Naoki Watanabe12. FRET-based measurements of tension Brenton D. Hoffman13. Mitochondria size Susanne Rafelski14. 3D multicolor super-resolution screening Ricardo Henriques15. Super-resolution measurements in vivo Pierre-François Lenne16. Single molecule measurements in cells with gold nanoprobes Cecile Leduc17. Single molecule imaging of motors in cells Iva M. Tolic and Vaishnavi Ananthanarayanan 18. Monitoring single gene expression Jonathan Chubb19. Plasma membrane wounding and repair Matthieu Piel20. Electrofusion of giant unilamellar vesicles to cells Kinneret Keren21. Host-pathogen interactions Pietro Cicuta22. Laser ablation for cortex tension measurements Stephan Grill23. AFM on plant cells Siobhan Braybrook24. Measuring and manipulating cellular volumes in fission yeast Nicolas Minc25. Analyzing bacterial movements Nicolas Biais26. Force measurements in tissues Otger Campas27. Video Force microscopy Wayne Brodland and Jim Veldhuis 28. Methods for rectifying cell motions in vitro: breaking symmetry using microfabrication and microfluidics Daniel Riveline
- ISBN: 978-0-12-801103-4
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 464
- Fecha Publicación: 15/01/2015
- Nº Volúmenes: 1
- Idioma: Inglés