sCongenital heart disease is the leading cause of infant death and affects approximately one in every 100 babies born in the United States. The study of cardiovascular development has acquired new momentum in last twenty years due tothe advancement of modern molecular biology and new available equipments and techniques. In Cardiovascular Development: Methods and Protocols expert researchers in the field in the field detail many of the methods which are now commonly used in the field of cardiovascular development research. These include methods and technique for using different organisms for cardiovascular developmental research, using cell and molecular biology methods to study cardiovascular development, as well as other available techniques for cardiovascular development research. Written in the highly successful Methods in Molecular Biology™series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproduciblelaboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiovascular Development: Methods and Protocols seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research including in vivo imaging and Bioinformatics. Covers state-of-the-art techniques in the field of cardiovascular development. Provides step-by-step detail essential for reproducible results. Containskey notes and implementation advice from the experts. INDICE: Use of Whole Embryo Culture for Studying Heart Development.-Quantifying Cardiac Functions in Embryonic and Adult Zebrafish.-Analysis of the Patterning of Cardiac Outflow Tract and Great Arteries with Angiography and Vascular Casting.-Morpholino Injection in Xenopus. -Chicken Chorioallantoic MembraneAngiogenesis Model.-Visualizing Vascular Networks in Zebrafish: An Introduction to Microangiography.-Whole-mount Confocal Microscopy for Vascular BranchingMorphogenesis.-Visualization of Mouse Embryo Angiogenesis by Fluorescence-based Staining.-Miniaturized Assays of Angiogenesis in Vitro.-Analysis of the Endocardial-to-Mesenchymal Transformation of Heart Valve Development by a Collagen Gel Culture Assay.-Quantification of Myocyte Chemotaxis: A role for FAK in Regulating Directional Motility.-Analysis of Neural Crest Cell Fate During Cardiovascular Development using Cre-activated lacZ /b-galactosidase Staining.-Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte.-Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung.-Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells.-Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization.-C-kit Expression Identifies Cardiac Precursor Cells.-Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols.-Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels.-Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups.-The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways.-Application of Atomic Force Microscopy Measurements on Cardiovascular Cells. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy.-Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in MicroarrayAnalysis.-Visual Data Mining of Co-expression Data to Set Research Prioritiesin Cardiac Development Research.-High-speed Confocal Imaging of Zebrafish Heart Development.-Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays.
- ISBN: 978-1-61779-522-0
- Editorial: Humana Press
- Encuadernacion: Cartoné
- Páginas: 325
- Fecha Publicación: 28/02/2012
- Nº Volúmenes: 1
- Idioma: Inglés