The Aptamers Ligands: Powerful Tools from Molecular Evolution

The Aptamers Ligands: Powerful Tools from Molecular Evolution discusses these short, single strand oligonucleotides that can fold in a three-dimensional conformation and how they can provide a precise molecular recognition of a given target. These nucleic acid based ligands are identified from large naive combinatorial oligonucleotide libraries using a well-established technology called SELEX (systematic evolution of ligands by exponential enrichment). This approach inspired from the natural evolution concept where an expected evolutionary pressure allows the selection of ligands with very specific properties that can meet specification requirements unobtainable for antibodies or derived ligands. Aptamers can be extremely specific towards their target molecule with high selectivity and proper affinity dissociation constants. Moreover, they can be selected to recognize a high diversity of molecules, from very small compounds, to large macromolecules, such as proteins. Provides the molecular evolution of aptamersIncludes a technological history on the subjectPresents analytic and purification applications INDICE: 1. Concepts of evolution and molecular evolution2. Definitions and history of aptamers3. The SELEX principles and its variations4. How to analyze a SELEX5. The chemical optimization of aptamers6. Biosensors and other analytical applications7. The aptamoaffinty8. Applications for in vivo imaging9. Ribozymes or natural aptamers10. Drug delivery applications11. Aptamer as direct therapeutic agent

• ISBN: 978-1-78548-213-7
• Editorial: ISTE Press - Elsevier
• Encuadernacion: Cartoné
• Páginas: 200
• Fecha Publicación: 01/02/2018
• Nº Volúmenes: 1
• Idioma: Inglés