Essential Enzyme Kinetics: A Textbook for Molecular Life Scientists
Purich, Daniel L.
Essentials of Enzyme Kinetics: A Textbook for Molecular Life Scientists discusses best-practice approaches for using site-directed mutagenesis to explore enzyme catalysis. The book presents a balance of initial-rate methods, fast reaction techniques, and kinetic isotope effect approaches, providing a must-have reference for bioscience workers and investigators who seek a better understanding of enzyme kinetics. Non-biochemists entering careers in Big Pharma will also benefit from the book's content as covered topics have become main drivers in small-molecule drug development. Offers practical information on how to work with enzymes and design experiments to identify new inhibitors or activatorsProvides detailed, step-by-step derivations of rate equations, showing tried-and-true ways to confirm that equations are correctIncludes 10-12 problems for each chapter INDICE: 1. Introduction 2. Basic Chemical Kinetics 3. Initial-Rate Kinetics of One-Substrate Enzymes 4. Measuring Initial Velocities of Enzyme-catalyzed Reactions 5. Multi-substrate Enzyme Kinetic Mechanisms 6. Fast Kinetic Techniques for Probing Enzyme-Catalyzed Reactions 7. Factors Affecting Enzyme Rates 8. Kinetic Isotope Effects 9. Inhibitor Effects on Enzyme-Catalyzed Reactions 10. Enzyme Cooperativity 11. Kinetics of Force-Generating Enzymes
- ISBN: 978-0-12-821808-2
- Editorial: Academic Press
- Encuadernacion: Rústica
- Páginas: 425
- Fecha Publicación: 01/06/2020
- Nº Volúmenes: 1
- Idioma: Inglés