Since the development of the concept of “proteomics, great progress has granted deeper insight into the cellular network, but the enormous range of proteinabundance, dynamics, and interactions leaves many further challenges in the field. In Proteomics: Methods and Protocols, experts in proteomic research havecontributed detailed techniques, which present an overview of the contemporary challenges and possibilities in the various areas of proteomics, as well as cutting-edge protocols that provide concrete examples for successful proteome analysis. Comprised of both overview articles and detailed protocols for core proteomics techniques Covers the hot topics in proteomics research, such as relative and absolute protein quantification, organellar and membrane studies aswell as PTM and protein interaction analysis Contains method descriptions forboth beginners and advanced proteomics researchers on electrophoresis, chromatography, and mass spectrometry INDICE: From the contents Introduction to Proteomics.- High-Resolution Two-Dimensional Electrophoresis.- Non-Classical 2-D Electrophoresis.- Protein Detection and Quantitation Technologies for Gel-Based Proteome Analysis.- MALDI MS.- Capillary Electrophoresis Coupled to Mass Spectrometry for Proteomic Profiling of Human Urine and Biomarker Discovery.- A Newcomer’s Guide to Nano-Liquid-Chromatography of Peptides.- Multidimensional Protein Identification Technology (MudPIT).- Characterization of Platelet Proteins Using Peptide Centric Proteomics.- Identification of the Molecular Composition of the 20S Proteasome ofMouse Intestine by High Resolution Mass Spectrometric Proteome Analysis.- Liquid Chromatography-Mass Spectrometry-Based Quantitative Proteomics.- iTRAQ-Labelling of In-Gel Digested Proteins for Relative Quantification.
- ISBN: 978-1-60761-156-1
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 390
- Fecha Publicación: 01/07/2009
- Nº Volúmenes: 1
- Idioma: Inglés