Angiogenesis is an important event in both physiological and pathological conditions. Under physiological conditions angiogenesis is tightly controlled, whereas increased production of angiogenic stimuli and/or reduced production of angiogenic inhibitors leads to abnormal neovascularization, such as occurs in cancer, chronic inflammatory disease, diabetic retinopathy, macular degeneration and cardiovascular disorders. Several genetic and epigenetic mechanisms are involved in the early development of the vascular system and there is an extensive literature on the genetic background and the molecular mechanisms responsible for blood vessel formation. Evidence is now emerging that blood vessels themselves have the ability to provide instructive regulatory signals to surrounding non-vascular target cells during organ development. Thus, endothelial cell signaling is currently believed to promote fundamental cues for cell fate specification, embryo patterning, organ differentiation and postnatal tissue remodeling. The aim of this book is to provide an overview of the most common assays for studying angiogenesis in vivo. Provides information on the most common assays to study angiogenesis in vivo, which will help the reader and the scientist in the choice of the most appropriate technique to utilizeWill appeal to those interested in angiogenesis as a normal and vital process in growth and development, as well as in wound healing and in the formation of granulation tissue, plus as a fundamental step in the transition of tumors from a benign state to a malignant one (leading to the use of angiogenesis inhibitors in the treatment of cancer) INDICE: The chorioallantoic membraneThe corneal micropocket assayThe zebrafish embryoThe sponge implant methodThe Matrigen implant assayIn vivo gene transfer with AAV vectorsIn vivo tumor models
- ISBN: 978-0-12-814020-8
- Editorial: Academic Press
- Encuadernacion: Rústica
- Páginas: 112
- Fecha Publicación: 01/11/2017
- Nº Volúmenes: 1
- Idioma: Inglés