Ligand-macromolecular interactions in drug discovery: methods and protocols

In Ligand-Macromolecular Interactions in Drug Discovery: Methods and Protocols, experts in the field highlight the main principles and methodologies currently utilized in the study of molecular interactions between compounds, either natural or synthetic, and complementary biological targets, within the scope of drug discovery. Beginning with a historical perspective of drug research focusing on the contribution of genomics, proteomics, high-throughput methods, and computational developments in drug discovery, the book then delves into highly detailed methods on topics such as NMR spectroscopy, compound library design and synthesis, chemical and biological microarrays, and many others. Writtenin the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and noteson troubleshooting and avoiding known pitfalls. Covers a wide range of techniques and assays for the design and screening of chemical and biological microarrays and libraries Comprehensive guide to developing, running, and analyzing ligand-macromolecule assays in high-throughput screening formats Readily reproducible techniques for key experiments in the search and analysis of bioactivecompounds Step-by-step laboratory instructions that ensure successful results INDICE: From the contents n Historical Overview of Drug Discovery.- LigandMacromolecule Interactions: Theoretical Principles of Molecular Recognition.-X-Ray Crystallography in Drug Discovery.- Virtual Screening of Compound Libraries.- Combinatorial Chemistry and the Synthesis of Compound Libraries.- Ligand-Based Nuclear Magnetic Resonance Screening Techniques.- Isothermal TitrationCalorimetry and Differential Scanning Calorimetry.- Adaptive Combinatorial Design of Focused Compound Libraries.- Chemical Microarrays: A New Tool for Discovery Enzyme Inhibitors.- Fluorescence Polarization and Time-Resolved Fluorescence Resonance Energy Transfer Techniques for PI3K Assays.- Small Molecule Protein Interaction Profiling with Functional Protein Microarrays.- Capillary Electrophoresis in Drug Discovery.- SPR in Drug Discovery: Searching Bioactive Compounds in Plant Extracts.

• ISBN: 978-1-60761-243-8
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 290
• Fecha Publicación: 01/09/2009
• Nº Volúmenes: 1
• Idioma: Inglés