This new volume of Methods in Cell Biology looks at building a cell from its component parts. Chapters cover such topics as engineering motor scaffolds, artificial cytoskeletons, interconnected droplet networks and artificial cells; building cytoskeletal systems and artificial actin cortex on pillar arrays; reconstituting membrane fission, actin bundles at membranes, actin cortex in droplets, dynein-dynactin mediated cargo transport, MinD systems, protein-lipid machines that transform membranes, protein interactions and signaling on artificial lipid bilayers. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studiesChapters are written by experts in the fieldCutting-edge material INDICE: 1. In Vitro Systems for the Study of Microtubule-Based Cell Polarity in Fission YeastNúria Taberner, Andries Lof, Sophie Roth, Dimitry Lamers, Hans Zeijlemaker and Marileen Dogterom2. Microtubules, MAPs, and Motor PatternsKasimira T. Stanhope and Jennifer L. Ross3. Self-Organization of Motors and Microtubules in Lipid-Monolayered DropletsHella Baumann and Thomas Surrey4. Reconstitution of Microtubule Based Motility Using Cell ExtractsSwathi Ayloo and Erika L. F. Holzbaur5. Building Cells for Quantitative, Live-cell Analyses of Collective Motor Protein FunctionsEric A. Kumar, David Tsao, Anand Radhakrishnan and Michael Diehl6. Reconstituting Cytoskeletal Contraction Events with Biomimetic Actin-Myosin Active gelsJosé Alvarado and Gijsje H. Koenderink7. Building an Artificial Actin Cortex on Microscopic Pillar ArraysR. Ayadi and W. H. Roos8. Triggering Actin Polymerization in Xenopus Egg Extracts from Phosphoinositide-Containing Lipid BilayersAstrid Walrant, Daniel S. Saxton, Guilherme Pereira Correia and Jennifer L. Gallop9. Reconstituting Geometry Modulated Protein Patterns in Membrane CompartmentsKatja Zieske and Petra Schwille10. Structural and Functional Studies of Membrane Remodeling MachinesRaghav Kalia, Nathaniel Talledge and Adam Frost11. Building Interconnected Membrane NetworksMatthew A. Holden12. Using Supported Bilayers to Study the Spatiotemporal Organization of Membrane Bound ProteinsPhuong A. Nguyen, Christine M. Field, Aaron C. Groen, Timothy J. Mitchison and Martin Loose 13. Reconstituting ParA/ParB-Mediated Transport of DNA CargoAnthony G. Vecchiarelli, James A. Taylor and Kiyoshi Mizuuchi14. Cell-Sized Liposomes that Mimic Cell Motility and the Cell CortexJoël Lemière, Kevin Carvalho and Cécile Sykes15. Reconstitution of Cortical Actin Networks Within Water-In-Oil EmulsionsEnas Abu Shah, Maya Malik-Garbi and Kinneret Keren16. Engineering Artificial Cells by Combining HeLa-Based Cell-Free Expression and Ultra-Thin Double Emulsion TemplateKenneth K.Y. Ho, Victoria L. Murray, Allen P. Liu17. Reconstitution of Proteins on Electroformed Giant Unilamellar VesiclesEva M. Schmid, David L. Richmond, and Daniel A. Fletcher18. Reconstituting SNARE-Mediated Membrane Fusion at the Single Liposome LevelVolker Kiessling, Binyong Liang and Lukas K. Tamm
- ISBN: 978-0-12-802450-8
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 362
- Fecha Publicación: 01/07/2015
- Nº Volúmenes: 1
- Idioma: Inglés