This
volume provides different methodologies for all classes of histone
deacetylases, which includes detailed procedures on Class I and II histone
deacetylase inhibitors, SIRT inhibitors, and bromodomain inhibitors. Histone
Deacetylases: Methods and Protocols is divided into four sections: Sections
A and B describe methodologies used to detect the activity, function, or
chromatin location of HDACs 1 through 11, with Section A discussing Class I and
Section B discussing class II histone deacetylases; Section C focuses on the
methodologies for cloning and characterizing the acetylation of SIRTs 1 through
7; and Section D describes methods related to histone deacetylase inhibitors. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols and tips on troubleshooting and
avoiding known pitfalls.
Thorough
and cutting-edge, Histone Deacetylases: Methods and Protocols, is a
valuable resource for investigators working on epigenetics, molecular biology,
and genetics.
- ISBN: 978-1-493-93665-6
- Editorial: Humana Press
- Encuadernacion: Cartoné
- Páginas: 300
- Fecha Publicación: 08/05/2016
- Nº Volúmenes: 1
- Idioma: Inglés