In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research focusing on stem cells. In Neural Cell Transplantation: Methods and Protocols, leading experts in the field examine tried-and-true laboratory techniques in order to supply scientists with a firm foundation upon which further advancements can bebased. Written in the highly successful Methods in Molecular Biology™ series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and Notes sections, which examine tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Neural Cell Transplantation: Methods and Protocols provides the most thorough and essential protocols that will allow new generations of neuroscientists to enter and contribute to this uniquely inspiring field. Provides concise introductory overviewsproviding a comprehensive background to the field Includes bench-to-bedside coverage spanning the whole spectrum of practical procedures from laboratory toclinic Written throughout by leading, active researchers in the field Describes detailed methodologies reproducible at the bench A chapter per technique, with no further reference-chasing INDICE: From the contents Neural Transplantation and Stem Cells.- Adult Stem Cells for the Treatment of Neurological Disease.- Human Trials for Neurodegenerative Disease.- Differentiation of Neuroepithelia from Human Embryonic Stem Cells.- Derivation of High Purity Oligodendroglial Progenitors.- Flow Cytometric Characterization of Neural Precursor Cells and their Progeny.- Isolation,Expansion and Differentiation of Adult Mammalian Neural Stem and Progenitor Cells Using the Neurosphere Assay.- Human Mesenchymal Stem Cell Culture for Neural Transplantation.- Umbilical Cord Blood Cells.- Animal Models of Neurodegenerative Diseases.- Animal Models of Multiple Sclerosis.- Transplantation of Oligodendrocyte Progenitor Cells in Animal Models of Leukodystrophies.- A Rat Middle Cerebral Artery Occlusion (MCAO) Model and Intravenous Cellular Delivery.
- ISBN: 978-1-60327-930-7
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 240
- Fecha Publicación: 01/05/2009
- Nº Volúmenes: 1
- Idioma: Inglés