Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry

Spatial Mapping of Biomolecules by Imaging Mass Spectrometry (IMS) introduces scientists to IMS and describes its many applications for diverse classes of biomolecules. It is divided into two major sections, the first being intended for beginners to build a foundation of IMS knowledge by introducing sample preparation, visualization guidelines, molecule identification, quantification, and software and hardware tools. The second, central part of the book contains individual chapters focused on each biomolecule class (sugars, polar metabolites, and so on). These chapters all include an introduction/background, review of complementary non-IMS techniques, a summary of successful IMS case studies with illustrative figures, a table (showing tissue, condition, ionization modality, matrix/solvent, sample prep, mass spectrometer, and reference), and future directions. This second part can be used by novices as well as by experts who are looking for a quick deep-dive into IMS for a specific class of molecule used in their research. Provides the introductory foundations of Imaging Mass Spectrometry for those new to the techniqueOrganized by molecule class to facilitate a quick deep-dive, allowing researchers to immediately apply these techniques to their workIncludes a table in every molecule class chapter summarizing the IMS experiments done for each class of molecules INDICE: Section I: Imaging Mass Spectrometry Basics 1. Introduction to imaging mass spectrometry 2. Sample preparation guidelines 3. Molecule identification strategies 4. Quantitation workflow in imaging mass spectrometry 5. Molecule visualization schemes (normalization, scale, color schemes) 6. Software 7. Hardware Section II: Biomolecule Mapping by Imaging Mass Spectrometry 8. Sugars 9. N-Glycan 10. Gangliosides 11. Amino Acids 12. Neuropeptides 13. Ceramides 14. Sphingomyelins 15. Phospholipids 16. Secondary plant metabolites 17. TCA cycle metabolites 18. Neurotransmitters 19. Drugs - examples 20.Steroids 21. ADP, ATP 22. Bacterial metabolites 23. Fatty acids 24. Triglyceride 25. Proteins

• ISBN: 978-0-12-818998-6
• Editorial: Elsevier
• Encuadernacion: Rústica
• Páginas: 300
• Fecha Publicación: 01/11/2020
• Nº Volúmenes: 1
• Idioma: Inglés